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Articles by Stephen H. Safe in JoVE
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages Wei Ying1, Patali S. Cheruku2, Fuller W. Bazer1, Stephen H. Safe3, Beiyan Zhou3 1Department of Animal Science, Texas A&M University, 2Department of Biology, Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.