Articles by Steven C. Nesbit in JoVE
Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle Maria Cano Garcia1, Steven C. Nesbit1, Chi C. Le2, James R. Dearworth Jr.1 1Department of Biology and Neuroscience Program, Lafayette College, 2Department of Information Technology, Computer Science, and Digital Media, Juniata College This protocol describes how to use an in vitro isolated turtle head preparation to measure the kinematics of their eye movements. After removal of the brain from the cranium, cranial nerves can be stimulated with currents to quantify rotations of the eye and changes in pupil sizes.
Other articles by Steven C. Nesbit on PubMed
Extracellular Recording of Light Responses from Optic Nerve Fibers and the Caudal Photoreceptor in the Crayfish Journal of Undergraduate Neuroscience Education : JUNE : a Publication of FUN, Faculty for Undergraduate Neuroscience. | Pubmed ID: 26557793 Few laboratory exercises have been developed using the crayfish as a model for teaching how neural processing is done by sensory organs that detect light stimuli. This article describes the dissection procedures and methods for conducting extracellular recording from light responses of both the optic nerve fibers found in the animal's eyestalk and from the caudal photoreceptor located in the ventral nerve cord. Instruction for ADInstruments' data acquisition system is also featured for the data collection and analysis of responses. The comparison provides students a unique view on how spike activities measured from neurons code image-forming and non-image-forming processes. Results from the exercise show longer latency and lower frequency of firing by the caudal photoreceptor compared to optic nerve fibers to demonstrate evidence of different functions. After students learn the dissection, recording procedure, and the functional anatomy, they can develop their own experiments to learn more about the photoreceptive mechanisms and the sensory integration of modalities by these light-responsive interneurons.