Articles by Steven L. Goodman in JoVE
利用胶囊对生物达标中病毒样品的阴性染色 Candace D. Blancett1, Mitchell K. Monninger1, Chrystal A. Nguessan1, Kathleen A. Kuehl1, Cynthia A. Rossi2, Scott P. Olschner2, Priscilla L. Williams2, Steven L. Goodman3, Mei G. Sun1 1Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC 该协议为负染色病毒样本提供了指导，可以很容易地用于BSL-2，-3或-4实验室。它包括使用创新的加工胶囊，其保护透射电子显微镜网格并且在生物达到的更湍流环境中为使用者提供更容易的处理。
Other articles by Steven L. Goodman on PubMed
新光漂剂的多光子激发的蛋白交联的三维亚微米： 牛血清白蛋白和 1 型胶原。 Photochemistry and Photobiology. Aug, 2002 | Pubmed ID: 12194208 我们报告的合成与光学表征的两个新光漂剂和证明其使用的多光子激发与蛋白质的三维形式自由制作。与交联类型 1 胶原的目标制定了这些试剂。此交联过程产生微米和亚微米大小尺度上的结构。玫瑰的孟加拉二异丙胺衍生物将组合成一个分子，减少反应动力学和提高交联效率的经典 photoactivator 和复合引发体系。玫瑰孟加拉单不是有效而成功生产胶原蛋白，从稳定结构在此衍生。二苯甲酮二聚体通过灵活二胺系绳连接也被合成。此激活器具有两个光化学无功组和非常高效的交联牛血清白蛋白和向窗体稳定、 鲁棒结构类型 1 胶原。这种方法是由传统二苯甲酮光化学交联各种各样的蛋白质比更灵活。物理性质差别很大，二苯甲酮与外观的一个新的、 较低能量吸收带 （水中的 lambda max 大约 370 nm） 和广泛，从可见发射带 （大约 500 牛米最大）。此吸收带区高度是溶致变色，暗示它出现，至少在部分中，从一个电荷转移互动。胶原蛋白是通常难以光化学，交叉链接，这里的结果表明这两个新的激活器将适合交联胶原蛋白和其他蛋白质如 de novo 大会的仿生组织支架材料的生物医学应用的其他形式。
Platelet Responses to Silicon-alloyed Pyrolytic Carbons Journal of Biomedical Materials Research. Part A. Oct, 2007 | Pubmed ID: 17380499 Pyrolytic carbon (PYC) containing approximately 7 wt % silicon is used in most clinical mechanical heart valves where it has demonstrated a high level of blood compatibility. The Si, present as SiC, is included since it is believed to enhance durability. However, it has been suggested that SiC reduces PYC blood compatibility. In the present study, PYC valve leaflets were prepared with low, conventional, and high levels of Si. The in vitro responses of human platelets to these materials were then quantified. Platelet responses were consistent with previous reports: Adherent platelets were extremely well spread, closely followed submicron contours, and formed very few aggregates or microthrombi-like structures. No significant differences with respect to the Si concentrations were observed for platelets adherent per unit area and the numbers of thrombi-like structures. Some differences were observed with platelet morphologies and the material surface covered with platelets, although these did not vary consistently with respect to Si concentration. These results indicate that lowering (or raising) the Si alloy concentration in PYC over a reasonable range (0.54-13.5 wt % as examined here) is unlikely to improve or otherwise alter the in vivo blood compatibility of this important clinical material.
Preparation of Viral Samples Within Biocontainment for Ultrastructural Analysis: Utilization of an Innovative Processing Capsule for Negative Staining Journal of Virological Methods. Dec, 2016 | Pubmed ID: 27751950 Transmission electron microscopy can be used to observe the ultrastructure of viruses and other microbial pathogens with nanometer resolution. In a transmission electron microscope (TEM), the image is created by passing an electron beam through a specimen with contrast generated by electron scattering from dense elements in the specimen. Viruses do not normally contain dense elements, so a negative stain that places dense heavy metal salts around the sample is added to create a dark border. To prepare a virus sample for a negative stain transmission electron microscopy, a virus suspension is applied to a TEM grid specimen support, which is a 3mm diameter fragile specimen screen coated with a few nanometers of plastic film. Then, deionized (dI) water rinses and a negative stain solution are applied to the grid. All infectious viruses must be handled in a biosafety cabinet (BSC) and many require a biocontainment laboratory environment. Staining viruses in biosafety levels (BSL) 3 and 4 is especially challenging because the support grids are small, fragile, and easily moved by air currents. In this study we evaluated a new device for negative staining viruses called mPrep/g capsule. It is a capsule that holds up to two TEM grids during all processing steps and for storage after staining is complete. This study reports that the mPrep/g capsule method is valid and effective to negative stain virus specimens, especially in high containment laboratory environments.