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Articles by Stuart MacLeod in JoVE
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping Ceres Kosuta1,2, Kate Daniel1, Devon L. Johnstone1,2, Kevin Mongeon1,2, Kevin Ban1,2, Sophie LeBlanc1, Stuart MacLeod1, Karim Et-Tahiry2, Marc Ekker2, Alex MacKenzie1, Izabella Pena1,2 1 Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.