In JoVE (1)
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Articles by Susana Pineda in JoVE
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR Sonia Apaza1, Susan Espetia1, Robert H. Gilman1,2, Sonia Montenegro3, Susana Pineda3, Fanny Herhold1, Romeo Pomari1, Margaret Kosek2, Nancy Vu1, Mayuko Saito1,2,4 1Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health, Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, 4University of California San Diego School of Medicine A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.