In JoVE (1)
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Articles by Susanne Carina van den Brink in JoVE
Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro Peter Baillie-Johnson1, Susanne Carina van den Brink1,2, Tina Balayo1, David Andrew Turner1, Alfonso Martinez Arias1 1Department of Genetics, University of Cambridge, 2Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences We have developed a protocol to generate aggregates of mouse embryonic stem cells that display self-organization, symmetry breaking and elongation paralleling axial development. This technique allows the study of axial developmental processes and the generation of cell types that are otherwise difficult to perform in monolayer culture.