Other Publications (1)
Articles by Suyog Yoganarasimha in JoVE
Caratterizzazione di leucociti-piastrine Rich fibrina, un biomateriale Novel Parthasarathy Madurantakam1, Suyog Yoganarasimha2, Fadi K. Hasan3 1Department of General Practice, School of Dentistry, Virginia Commonwealth University, 2Department of Biomedical Engineering, Virginia Commonwealth University, 3Department of Periodontics, School of Dentistry, Virginia Commonwealth University
Other articles by Suyog Yoganarasimha on PubMed
Peracetic Acid: a Practical Agent for Sterilizing Heat-labile Polymeric Tissue-engineering Scaffolds Tissue Engineering. Part C, Methods. Sep, 2014 | Pubmed ID: 24341350 Advanced biomaterials and sophisticated processing technologies aim at fabricating tissue-engineering scaffolds that can predictably interact within a biological environment at the cellular level. Sterilization of such scaffolds is at the core of patient safety and is an important regulatory issue that needs to be addressed before clinical translation. In addition, it is crucial that meticulously engineered micro- and nano- structures are preserved after sterilization. Conventional sterilization methods involving heat, steam, and radiation are not compatible with engineered polymeric systems because of scaffold degradation and loss of architecture. Using electrospun scaffolds made from polycaprolactone, a low melting polymer, and employing spores of Bacillus atrophaeus as biological indicators, we compared ethylene oxide, autoclaving and 80% ethanol to a known chemical sterilant, peracetic acid (PAA), for their ability to sterilize as well as their effects on scaffold properties. PAA diluted in 20% ethanol to 1000 ppm or above sterilized electrospun scaffolds in 15 min at room temperature while maintaining nano-architecture and mechanical properties. Scaffolds treated with PAA at 5000 ppm were rendered hydrophilic, with contact angles reduced to 0°. Therefore, PAA can provide economical, rapid, and effective sterilization of heat-sensitive polymeric electrospun scaffolds that are used in tissue engineering.