Articles by Sylvain Coulombe in JoVE
Permeabilizzazione delle cellule Aderito utilizzo di un gas inerte Jet Scott Cooper1, Paul Jonak1, Guillaume Chouinard-Pelletier1, Sylvain Coulombe1, Elizabeth Jones1, Richard L. Leask1,2 1Chemical Engineering, McGill University, 2Montreal Heart Institute Questo protocollo descrive un metodo per la permeabilizzazione temporanea di cellule aderenti mediante un getto di gas inerte. Questa tecnica facilita il trasferimento di materiale genetico e biomolecole in cellule di mammifero aderenti mediante l'utilizzo delle forze meccaniche di distruggere la membrana plasmatica.
Other articles by Sylvain Coulombe on PubMed
Use of Inert Gas Jets to Measure the Forces Required for Mechanical Gene Transfection Biomedical Engineering Online. 2012 | Pubmed ID: 22963645 Transferring genes and drugs into cells is central to how we now study, identify and treat diseases. Several non-viral gene therapy methods that rely on the mechanical disruption of the plasma membrane have been proposed, but the success of these methods has been limited due to a lack of understanding of the mechanical parameters that lead to cell membrane permeability.
Feasibility of Nanofluid-based Optical Filters Applied Optics. Mar, 2013 | Pubmed ID: 23458793 In this article we report recent modeling and design work indicating that mixtures of nanoparticles in liquids can be used as an alternative to conventional optical filters. The major motivation for creating liquid optical filters is that they can be pumped in and out of a system to meet transient needs in an application. To demonstrate the versatility of this new class of filters, we present the design of nanofluids for use as long-pass, short-pass, and bandpass optical filters using a simple Monte Carlo optimization procedure. With relatively simple mixtures, we achieve filters with