Articles by Sylvain Coulombe in JoVE
불활성 가스 제트를 사용하여 붙음 세포의 Permeabilization Scott Cooper1, Paul Jonak1, Guillaume Chouinard-Pelletier1, Sylvain Coulombe1, Elizabeth Jones1, Richard L. Leask1,2 1Chemical Engineering, McGill University, 2Montreal Heart Institute 이 프로토콜은 불활성 가스 제트를 이용한 부착 세포의 임시 permeabilization위한 방법을 설명한다. 이 방법은 세포막을 방해하는 기계적 힘의 이용에 의한 부착 성 포유 동물 세포에 유전 물질과 생체 분자의 전달을 용이하게한다.
Other articles by Sylvain Coulombe on PubMed
Use of Inert Gas Jets to Measure the Forces Required for Mechanical Gene Transfection Biomedical Engineering Online. 2012 | Pubmed ID: 22963645 Transferring genes and drugs into cells is central to how we now study, identify and treat diseases. Several non-viral gene therapy methods that rely on the mechanical disruption of the plasma membrane have been proposed, but the success of these methods has been limited due to a lack of understanding of the mechanical parameters that lead to cell membrane permeability.
Feasibility of Nanofluid-based Optical Filters Applied Optics. Mar, 2013 | Pubmed ID: 23458793 In this article we report recent modeling and design work indicating that mixtures of nanoparticles in liquids can be used as an alternative to conventional optical filters. The major motivation for creating liquid optical filters is that they can be pumped in and out of a system to meet transient needs in an application. To demonstrate the versatility of this new class of filters, we present the design of nanofluids for use as long-pass, short-pass, and bandpass optical filters using a simple Monte Carlo optimization procedure. With relatively simple mixtures, we achieve filters with