Taisuke Tomita Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences The University of Tokyo Biography Publications Institution JoVE Articles Taisuke Tomita has not added a biography. If you are Taisuke Tomita and would like to personalize this page please email our Author Liaison for assistance. Publications Activation of γ-secretase Trimming Activity by Topological Changes of Transmembrane Domain 1 of Presenilin 1 The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 2017 | Pubmed ID: 29118109 Memantine Inhibits β-amyloid Aggregation and Disassembles Preformed β-amyloid Aggregates Biochemical and Biophysical Research Communications. Nov, 2017 | Pubmed ID: 28917837 Two Novel Mutations in the First Transmembrane Domain of Presenilin1 Cause Young-Onset Alzheimer's Disease Journal of Alzheimer's Disease : JAD. 2017 | Pubmed ID: 28550247 Dysregulated Metabolism of the Amyloid-β Protein and Therapeutic Approaches in Alzheimer Disease Journal of Cellular Biochemistry. Dec, 2017 | Pubmed ID: 28488760 The Crystal Structure of Mouse LC3B in Complex with the FYCO1 LIR Reveals the Importance of the Flanking Region of the LIR Motif Acta Crystallographica. Section F, Structural Biology Communications. 03, 2017 | Pubmed ID: 28291748 Memantine Reduces the Production of Amyloid-β Peptides Through Modulation of Amyloid Precursor Protein Trafficking European Journal of Pharmacology. Mar, 2017 | Pubmed ID: 28167259 Aberrant Proteolytic Processing and Therapeutic Strategies in Alzheimer Disease Advances in Biological Regulation. 05, 2017 | Pubmed ID: 28082052 Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility Method Methods in Enzymology. 2017 | Pubmed ID: 28065263 Molecular Mechanisms of the Genetic Risk Factors in Pathogenesis of Alzheimer Disease Frontiers in Bioscience (Landmark Edition). Jan, 2017 | Pubmed ID: 27814610 Partial Loss of CALM Function Reduces Aβ42 Production and Amyloid Deposition in Vivo Human Molecular Genetics. Sep, 2016 | Pubmed ID: 27466196 Cell-free Methods to Produce Structurally Intact Mammalian Membrane Proteins Scientific Reports. Jul, 2016 | Pubmed ID: 27465719 BIN1 Regulates BACE1 Intracellular Trafficking and Amyloid-β Production Human Molecular Genetics. Jul, 2016 | Pubmed ID: 27179792 A Novel Non-canonical Notch Signaling Regulates Expression of Synaptic Vesicle Proteins in Excitatory Neurons Scientific Reports. Apr, 2016 | Pubmed ID: 27040987 Retromer and Rab2-dependent Trafficking Mediate PS1 Degradation by Proteasomes in Endocytic Disturbance Journal of Neurochemistry. 05, 2016 | Pubmed ID: 26896628 Conformational Changes in Transmembrane Domain 4 of Presenilin 1 Are Associated with Altered Amyloid-β 42 Production The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2016 | Pubmed ID: 26818522 Suppressor Mutations for Presenilin 1 Familial Alzheimer Disease Mutants Modulate γ-Secretase Activities The Journal of Biological Chemistry. Jan, 2016 | Pubmed ID: 26559975 Cooperative Roles of Hydrophilic Loop 1 and the C-terminus of Presenilin 1 in the Substrate-gating Mechanism of γ-secretase The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Feb, 2015 | Pubmed ID: 25673856 Synthetic Ceramide Analogues Increase Amyloid-β 42 Production by Modulating γ-secretase Activity Biochemical and Biophysical Research Communications. Feb, 2015 | Pubmed ID: 25545059 Molecular Mechanism of Intramembrane Proteolysis by γ-secretase Journal of Biochemistry. Oct, 2014 | Pubmed ID: 25108625 Allosteric Regulation of γ-secretase Activity by a Phenylimidazole-type γ-secretase Modulator Proceedings of the National Academy of Sciences of the United States of America. Jul, 2014 | Pubmed ID: 25009180 Decreased CALM Expression Reduces Aβ42 to Total Aβ Ratio Through Clathrin-mediated Endocytosis of γ-secretase Nature Communications. Feb, 2014 | Pubmed ID: 24577224 Binding of Longer Aβ to Transmembrane Domain 1 of Presenilin 1 Impacts on Aβ42 Generation Molecular Neurodegeneration. Jan, 2014 | Pubmed ID: 24410857 Attenuation of the Aggregation and Neurotoxicity of Amyloid-β Peptides by Catalytic Photooxygenation Angewandte Chemie (International Ed. in English). Jan, 2014 | Pubmed ID: 24339209 Structural Interactions Between Inhibitor and Substrate Docking Sites Give Insight into Mechanisms of Human PS1 Complexes Structure (London, England : 1993). Jan, 2014 | Pubmed ID: 24210759 [Alzheimer Disease: Experimental Approaches to Genetic and Environmental Risk Factors] Rinsho Shinkeigaku = Clinical Neurology. 2013 | Pubmed ID: 24291845 Experimental Detection of Proteolytic Activity in a Signal Peptide Peptidase of Arabidopsis Thaliana BMC Biochemistry. Jul, 2013 | Pubmed ID: 23829174 Protein Trafficking and Maturation Regulate Intramembrane Proteolysis Biochimica Et Biophysica Acta. Dec, 2013 | Pubmed ID: 23770323 γ -Secretase Pharmacology: What Pharmacology Will Work for Alzheimer's Disease? International Journal of Alzheimer's Disease. 2013 | Pubmed ID: 23710414 FTY720/fingolimod, a Sphingosine Analogue, Reduces Amyloid-β Production in Neurons PloS One. 2013 | Pubmed ID: 23667698 Structural Biology of Presenilins and Signal Peptide Peptidases The Journal of Biological Chemistry. May, 2013 | Pubmed ID: 23585568 Inhibition of γ-secretase Activity by a Monoclonal Antibody Against the Extracellular Hydrophilic Loop of Presenilin 1 Biochemistry. Jan, 2013 | Pubmed ID: 23210549 [Development of Alzheimer's Disease Treatment Based on the Molecular Mechanism of γ-secretase Activity] Rinsho Shinkeigaku = Clinical Neurology. 2012 | Pubmed ID: 23196551 Activity-dependent Proteolytic Cleavage of Neuroligin-1 Neuron. Oct, 2012 | Pubmed ID: 23083742 Contribution of the γ-secretase Subunits to the Formation of Catalytic Pore of Presenilin 1 Protein The Journal of Biological Chemistry. Jul, 2012 | Pubmed ID: 22689582 Phenylpiperidine-type γ-secretase Modulators Target the Transmembrane Domain 1 of Presenilin 1 The EMBO Journal. Oct, 2011 | Pubmed ID: 22002539 Three-dimensional Structure of the Signal Peptide Peptidase The Journal of Biological Chemistry. Jul, 2011 | Pubmed ID: 21636854 BACE1 Activity is Modulated by Cell-associated Sphingosine-1-phosphate The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. May, 2011 | Pubmed ID: 21543615 Selectivity to Amyloid-β Precursor Protein Cleavage Provides Hope Against Alzheimer's Alzheimer's Research & Therapy. Mar, 2011 | Pubmed ID: 21418547 Participation of Transmembrane Domain 1 of Presenilin 1 in the Catalytic Pore Structure of the γ-secretase The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 2010 | Pubmed ID: 21106832 [Molecular Targeted Therapy in Alzheimer Disease] Nihon Rinsho. Japanese Journal of Clinical Medicine. Oct, 2010 | Pubmed ID: 20954338 Functional Analysis of the Transmembrane Domains of Presenilin 1: Participation of Transmembrane Domains 2 and 6 in the Formation of Initial Substrate-binding Site of Gamma-secretase The Journal of Biological Chemistry. Jun, 2010 | Pubmed ID: 20418378 [Structure and Function Analysis of Gamma-secretase Complex Toward Development of Therapeutics for Alzheimer's Disease] Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme. Sep, 2009 | Pubmed ID: 21089607 Development of Photoaffinity Probes for Gamma-secretase Equipped with a Nitrobenzenesulfonamide-type Cleavable Linker Bioorganic & Medicinal Chemistry Letters. Dec, 2009 | Pubmed ID: 19892551 [Biology and Pathobiology of the Intramembrane Proteolysis by Gamma-secretase] Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme. Oct, 2009 | Pubmed ID: 19827607 Single Chain Variable Fragment Against Nicastrin Inhibits the Gamma-secretase Activity The Journal of Biological Chemistry. Oct, 2009 | Pubmed ID: 19684016 Secretase Inhibitors and Modulators for Alzheimer's Disease Treatment Expert Review of Neurotherapeutics. May, 2009 | Pubmed ID: 19402777 S-palmitoylation of Gamma-secretase Subunits Nicastrin and APH-1 The Journal of Biological Chemistry. Jan, 2009 | Pubmed ID: 19028695 The C-terminal PAL Motif and Transmembrane Domain 9 of Presenilin 1 Are Involved in the Formation of the Catalytic Pore of the Gamma-secretase The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jun, 2008 | Pubmed ID: 18550769 At the Frontline of Alzheimer's Disease Treatment: Gamma-secretase Inhibitor/modulator Mechanism Naunyn-Schmiedeberg's Archives of Pharmacology. Jun, 2008 | Pubmed ID: 18038124 Abeta42 Overproduction Associated with Structural Changes in the Catalytic Pore of Gamma-secretase: Common Effects of Pen-2 N-terminal Elongation and Fenofibrate The Journal of Biological Chemistry. Apr, 2007 | Pubmed ID: 17329245 Structure of the Catalytic Pore of Gamma-secretase Probed by the Accessibility of Substituted Cysteines The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 2006 | Pubmed ID: 17108181 Presenilin-dependent Intramembrane Cleavage of Ephrin-B1 Molecular Neurodegeneration. Jun, 2006 | Pubmed ID: 16930449 C-terminal Fragment of Presenilin is the Molecular Target of a Dipeptidic Gamma-secretase-specific Inhibitor DAPT (N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine T-butyl Ester) The Journal of Biological Chemistry. May, 2006 | Pubmed ID: 16569643 Gamma-secretase As a Therapeutic Target for Treatment of Alzheimer's Disease Current Pharmaceutical Design. 2006 | Pubmed ID: 16472155 Association of Active Gamma-secretase Complex with Lipid Rafts Journal of Lipid Research. May, 2005 | Pubmed ID: 15716592 Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag Genta Ito1, Taisuke Tomita1,2 1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo JoVE 56688 Biochemistry
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag Genta Ito1, Taisuke Tomita1,2 1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo JoVE 56688 Biochemistry
