Articles by Takashi Taguchi in JoVE
Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats John R. Wagner1, Takashi Taguchi1, Jane Y. Cho2, Chandrashekhar Charavaryamath3, Dominique J. Griffon1 1College of Veterinary Medicine, Western University of Health Sciences, 2Applied Medical, 3College of Veterinary Medicine, Iowa State University This paper describes the preparation and evaluation of umbilical cord matrix-derived mesenchymal stem cells spheroids with a bilateral patellar tendon defect model in a rat. This model was associated with an acceptable morbidity and was found to detect differences between untreated and treated tendons, and between the two treatments tested.
Other articles by Takashi Taguchi on PubMed
Influence of Hypoxia on the Stemness of Umbilical Cord Matrix-derived Mesenchymal Stem Cells Cultured on Chitosan Films Journal of Biomedical Materials Research. Part B, Applied Biomaterials. | Pubmed ID: 28188976 Chitosan is attractive as a substrate for stem cell expansion because it improves stemness through formation of spheroids. Hypoxia has also been proposed as a strategy to enhance stemness and survival of stem cells after in vivo implantation. This study was therefore designed to evaluate the influence of hypoxia on chitosan-induced behavior of stem cells. Umbilical cord matrix-derived stem cells were cultured on chitosan film or standard plate under normoxia and hypoxia, for 3 and 7 days. Based on immunophenotyping, chitosan strongly suppresses the expression of CD90 and CD105 cell surface markers, changes partially reversed by combined exposure to hypoxia. Hypoxia generally increased the volume and number of spheroids formed on chitosan, but the cellularity of cultures on chitosan films remained lower than that of standard plates. After 7 days of culture, the expression of stemness related genes (Oct4, Sox2, and Nanog) was best stimulated by combined exposure to chitosan and hypoxia. Based on our results, conditioning stem cells for 7 days on chitosan films under hypoxic conditions is recommended to enhance the stemness of stem cells, and minimize cell loss due to lack of attachment on chitosan. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 501-511, 2018.