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Articles by Takeharu Morishita in JoVE
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate Makoto Kondo1,2, Takamichi Izumi1,2, Nao Fujieda1,2, Atsushi Kondo1,2, Takeharu Morishita1,2, Hirokazu Matsushita1, Kazuhiro Kakimi1 1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.