In JoVE (1)
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Articles by Taylor C.M. Harkness in JoVE
Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways André O. Hudson1, Taylor C.M. Harkness1, Michael A. Savka1 1Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology The validation of enzymatic activities involved in biochemical pathways can be elucidated using functional complementation analysis (FCA). Described in this manuscript is the FCA assay demonstrating the enzymatic activity of enzymes involved in the metabolism of amino acids, bacterial stringent response and bacterial peptidoglycan biosynthesis.