Articles by Teiko Shibata-Seki in JoVE
Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array Yasumasa Mashimo1,2, Momoko Yoshioka1, Yumie Tokunaga1, Christopher Fockenberg1, Shiho Terada1, Yoshie Koyama1, Teiko Shibata-Seki2, Koki Yoshimoto1, Risako Sakai1, Hayase Hakariya1, Li Liu1, Toshihiro Akaike3, Eiry Kobatake2, Siew-Eng How4, Motonari Uesugi1,5, Yong Chen1,6, Ken-ichiro Kamei1 1Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 5Institute for Chemical Research, Kyoto University, 6Ecole Normale Supérieure This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.
Other articles by Teiko Shibata-Seki on PubMed
AFM Characterization of Chemically Treated Corneal Cells Analytical and Bioanalytical Chemistry. Mar, 2015 | Pubmed ID: 25633218 We present a characterization of chemically treated cells using atomic force microscopy (AFM) which can observe changes in morphology and elasticity of cells. Since AFM has the significant advantage that it does not require fixation of samples, the method is simple and can capture various properties of living cells. In this study, corneal epithelial and endothelial cells were examined. The topography images of the corneal cells without glutaraldehyde (GA) fixation were successfully obtained. The images showed a natural three-dimensional shape of these cells, which scanning electron microscope (SEM) images could not provide. The AFM images of GA-fixed cells were taken and compared with a SEM image reported in the literature. Our results show that longer time for GA fixation makes the surface of the corneal endothelial tissue stiffer. Also, longer treatment results in relatively large structural variation in samples. Combined with conventional histochemical methods, this approach helps us gain an overall understanding of the influence of such chemical treatment.
Microfluidic-Nanofiber Hybrid Array for Screening of Cellular Microenvironments Small (Weinheim an Der Bergstrasse, Germany). May, 2017 | Pubmed ID: 28272774 Cellular microenvironments are generally sophisticated, but crucial for regulating the functions of human pluripotent stem cells (hPSCs). Despite tremendous effort in this field, the correlation between the environmental factors-especially the extracellular matrix and soluble cell factors-and the desired cellular functions remains largely unknown because of the lack of appropriate tools to recapitulate in vivo conditions and/or simultaneously evaluate the interplay of different environment factors. Here, a combinatorial platform is developed with integrated microfluidic channels and nanofibers, associated with a method of high-content single-cell analysis, to study the effects of environmental factors on stem cell phenotype. Particular attention is paid to the dependence of hPSC short-term self-renewal on the density and composition of extracellular matrices and initial cell seeding densities. Thus, this combinatorial approach provides insights into the underlying chemical and physical mechanisms that govern stem cell fate decisions.