In JoVE (1)
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Articles by Thomas P. Karbanowicz in JoVE
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells Thomas P. Karbanowicz1, Ala Lew-Tabor1,2, Manuel Rodriguez Valle1 1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.