In JoVE (1)
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Articles by Thomas N. Rösner in JoVE
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection Eric K. Hubner1,2, Christian Lechler1, Thomas N. Rösner1, Birgit Kohnke-Ertel1, Roland M. Schmid1, Ursula Ehmer1 1Department of Medicine II, Klinikum rechts der Isar, Technische Universität München, 2Department of Pneumology, Center for Medicine, Medical Center University of Freiburg Hydrodynamic tail vein injection of transposon-based integration vectors enables stable transfection of murine hepatocytes in vivo. Here, we present a practical protocol for transfection systems that enables the long-term constitutive expression of a single transgene or combined constitutive and doxycycline-inducible expression of a transgene or miR-shRNA in the liver.