In JoVE (1)
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Articles by Thomas Piers in JoVE
Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester Joseph Jebelli*1, Thomas Piers*2, Jennifer Pocock3 1Department of Neurology, University of Washington, 2Therapeutic Innovation Group, School of Life and Medical Sciences, University College London, 3Department of Neuroinflammation, University College London Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.