Articles by Tiffany Scott-Horton in JoVE
Pyrosequencing: Doğru Genotiplendirme için Basit Bir Yöntem Cristi King1, Tiffany Scott-Horton1 1Department of Internal Medicine, Washington University in St. Louis Pyrosequencing (R) polimorfizmleri analiz için kullanılan, bugüne kadarki en kapsamlı ama basit yöntemlerle biridir. Bu yöntem, birçok klinik olarak ilgili polimorfizmleri dahil olmak üzere, hızlı ve verimli bir tek nükleotid polimorfizmi değerlendirme yol açmıştır. Pyrosequencing tekniği ve metodoloji açıklanmıştır.
Other articles by Tiffany Scott-Horton on PubMed
Hoosier Oncology Group Randomized Phase II Study of Docetaxel, Vinorelbine, and Estramustine in Combination in Hormone-refractory Prostate Cancer with Pharmacogenetic Survival Analysis Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. Oct, 2006 | Pubmed ID: 17062685 To determine the safety and efficacy of two docetaxel doublets in hormone-refractory prostate cancer (HRPC) patients and to examine the prognostic role of polymorphisms in host genes important to docetaxel metabolism and transport.
Pyrosequencing: a Simple Method for Accurate Genotyping Methods in Molecular Biology (Clifton, N.J.). 2007 | Pubmed ID: 17185756 Pharmacogenetic research benefits first-hand from the abundance of information provided by the completion of the Human Genome Project. With such a tremendous amount of data available comes an explosion of genotyping methods. Pyrosequencing is one of the most thorough yet simple methods to date used to analyze polymorphisms. It also has the ability to identify tri-allelic, indels, short-repeat polymorphisms, along with determining allele percentages for methylation or pooled sample assessment. In addition, there is a standardized control sequence that provides internal quality control. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained in this chapter.
Methotrexate (MTX) Pathway Gene Polymorphisms and Their Effects on MTX Toxicity in Caucasian and African American Patients with Rheumatoid Arthritis The Journal of Rheumatology. Apr, 2008 | Pubmed ID: 18381794 Research has examined the association of folate-dependent gene polymorphisms with methotrexate (MTX) toxicity in racially homogenous patients with rheumatoid arthritis (RA). We examined the influence of MTX transporter gene polymorphisms on MTX toxicity in 2 racial groups of patients with RA.