Other Publications (1)
Articles by Tik Nga Tong in JoVE
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis Tik Nga Tong1, Donald R. Branch1,2 1Department of Laboratory Medicine and Pathobiology, University of Toronto, 2Centre for Innovation, Canadian Blood Services The monocyte monolayer assay (MMA) is an in vitro assay that utilizes isolated primary monocytes obtained from mammalian peripheral whole blood to evaluate Fcγ receptor (FcγR)-mediated phagocytosis.
Other articles by Tik Nga Tong on PubMed
Optimal Conditions for the Performance of a Monocyte Monolayer Assay Transfusion. Aug, 2016 | Pubmed ID: 27546234 Various versions of the monocyte monolayer assay (MMA) have been used to assess clinical significance of red blood cell (RBC) alloantibodies in transfusion for more than 35 years. However, the optimal conditions, including anticoagulant used for whole blood samples, temperature and duration of storage, and optimal pH for assessing the response of monocytes to antibody-bound RBCs, have never been clearly delineated.