Other Publications (1)
Articles by Timothy J. Tyree in JoVE
Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures Chen Shen*1, Ethan F. Julius*1, Timothy J. Tyree*1, Ritwik Dan1, David W. Moreau2, Robert Thorne2 1Cornell University, 2Physics Department, Cornell University A protocol for determining the vitreous phase densities of micro- to pico-liter size drops of aqueous mixtures at cryogenic temperatures is described.
Other articles by Timothy J. Tyree on PubMed
Thermal Contraction of Aqueous Glycerol and Ethylene Glycol Solutions for Optimized Protein-crystal Cryoprotection Acta Crystallographica. Section D, Structural Biology. Jun, 2016 | Pubmed ID: 27303794 The thermal contraction of aqueous cryoprotectant solutions on cooling to cryogenic temperatures is of practical importance in protein cryocrystallography and in biological cryopreservation. In the former case, differential contraction on cooling of protein molecules and their lattice relative to that of the internal and surrounding solvent may lead to crystal damage and the degradation of crystal diffraction properties. Here, the amorphous phase densities of aqueous solutions of glycerol and ethylene glycol at T = 77 K have been determined. Densities with accuracies of