Articles by Todd C. Lorenz in JoVE
Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies Todd C. Lorenz1 1Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.