Articles by Tracy A. Hookway in JoVE
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds Hannah A. Strobel1, Elizabeth L. Calamari1, Brittany Alphonse1, Tracy A. Hookway1,2, Marsha W. Rolle1 1Biomedical Engineering, Worcester Polytechnic Institute, 2Gladstone Institute for Cardiovascular Disease This protocol describes a platform for fabricating self-assembled tissue rings in variable sizes using a customized 3D-printed plastic mold. PDMS negatives are cured in the 3D-printed mold; then agarose is cast in the cured PDMS negatives. Cells are seeded into the resulting agarose wells where they aggregate into tissue rings.
Other articles by Tracy A. Hookway on PubMed
Self-assembled Smooth Muscle Cell Tissue Rings Exhibit Greater Tensile Strength Than Cell-seeded Fibrin or Collagen Gel Rings Journal of Biomedical Materials Research. Part A. | Pubmed ID: 22865465 In this study, we created self-assembled smooth muscle cell (SMC) tissue rings (comprised entirely of cells and cell-derived matrix; CDM) and compared their structure and material properties with tissue rings created from SMC-seeded fibrin or collagen gels. All tissue rings were cultured statically for 7 days in supplemented growth medium (with ε-amino caproic acid, ascorbic acid, and insulin-transferrin-selenium), prior to uniaxial tensile testing and histology. Self-assembled CDM rings exhibited ultimate tensile strength and stiffness values that were two-fold higher than fibrin gel and collagen gel rings. Tensile testing of CDM, fibrin gel and collagen gel rings treated with deionized water to lyse cells showed little to no change in mechanical properties relative to untreated ring samples, indicating that the ECM dominates the measured ring mechanics. In addition, CDM rings cultured in supplemented growth medium were significantly stronger than CDM rings cultured in standard, unsupplemented growth medium. These results illustrate the potential utility of self-assembled cell rings as model CDM constructs for tissue engineering and biomechanical analysis of ECM material properties.