In JoVE (1)
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Articles by Tyson J. Edwards in JoVE
In vivo Laser Axotomy in C. elegans Alexandra B. Byrne*1, Tyson J. Edwards*1, Marc Hammarlund1 1Department of Genetics, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.