Articles by Vaibhav Murthy in JoVE
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy Vaibhav Murthy*1, Dalton Dacus*1, Monica Gamez2, Changkun Hu1, Sebastian O. Wendel1, Jazmine Snow1, Andrew Kahn1, Stephen H. Walterhouse1, Nicholas A. Wallace1 1Division of Biology, Kansas State University, 2Bristol Medical School, Translational Health Sciences, University of Bristol Repair of double-strand DNA breaks is a dynamic process, requiring not only formation of repair complexes at the breaks, but also their resolution after the lesion is addressed. Here, we use immunofluorescence microscopy for transient and long-lasting double-stranded breaks as a tool to dissect this genome maintenance mechanism.
Other articles by Vaibhav Murthy on PubMed
Engineering the RNA-Nanobio Interface Bioengineering (Basel, Switzerland). | Pubmed ID: 28952492 RNA nanotechnology is attracting a great deal of attention recently. As the multiple roles that RNA plays in molecular biology and physiological regulation become clearer, there are many opportunities for engineering RNA-Nanoparticle Complexes (RNA-NPCs). The high "engineerability" of RNA-NPCs comes from the ability to modify the RNA and NP chemistry. For example, the NP can be derived from materials with anticancer activity and the RNA delivered by it, designed to target cell signaling pathways that contribute to the molecular basis of these diseases. Despite this rapid advancement and the availability of new quantification and characterization techniques, a key challenge is to develop a better understanding of the RNA-nanobio interface; that is, the interactions of RNA with NP (RNA-nanobio interface) and how that impacts the structure, function, delivery, and activity of the RNA. Here, we attempt to summarize the state-of-the-art in this new and exciting field, and to lay out potential directions for bioengineering research on RNA-NPCs.