In JoVE (1)
Articles by Varda S. Sardesai in JoVE
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue Rebecca A. Pelekanos1, Varda S. Sardesai1, Kathryn Futrega2, William B. Lott2, Michael Kuhn2, Michael R. Doran2,3 1UQ Centre for Clinical Research, The University of Queensland, 2Translational Research Institute, Queensland University of Technology, 3Translational Research Institute, Mater Medical Research - University of Queensland Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.
Other articles by Varda S. Sardesai on PubMed
Intracellular Trafficking and Endocytosis of CXCR4 in Fetal Mesenchymal Stem/stromal Cells BMC Cell Biology. 2014 | Pubmed ID: 24885150 Fetal mesenchymal stem/stromal cells (MSC) represent a developmentally-advantageous cell type with translational potential.To enhance adult MSC migration, studies have focussed on the role of the chemokine receptor CXCR4 and its ligand SDF-1 (CXCL12), but more recent work implicates an intricate system of CXCR4 receptor dimerization, intracellular localization, multiple ligands, splice variants and nuclear accumulation. We investigated the intracellular localization of CXCR4 in fetal bone marrow-derived MSC and role of intracellular trafficking in CXCR4 surface expression and function.
Rapid Method for Growth Hormone Receptor Exon 3 Delete (GHRd3) SNP Genotyping from Archival Human Placental Samples Endocrine. Aug, 2015 | Pubmed ID: 26067082 Analysis of archival samples from cohorts of pregnant women may be key to discovering prognosticators of stillbirth and pregnancy/perinatal complications. Growth hormone (GH) and its receptor (GHR) are pivotal in feto-placental development and pregnancy maintenance. We report a rapid, optimized method for genotyping the GHR full-length versus exon 3-deleted isoform (GHRd3). TaqMan single nucleotide polymorphism (SNP) genotyping proved superior to standard multiplex polymerase chain reaction (PCR) in allele detection and GHR genotyping from archived samples, including those with poor genomic deoxyribonucleic acid quality/quantity such as formalin fixed, paraffin embedded, blood, and serum. Furthermore, this assay is suitable for high through put 96 or 384-well plate quantitative PCR machines with automated genotype calling software. The TaqMan genotyping assay can increase the data obtained from precious archival human samples.