In JoVE (1)
Other Publications (0)
Articles by Vincent Ebacher in JoVE
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System Candice C. Poon1, Vincent Ebacher2, Katherine Liu1, Voon Wee Yong*1,3, John James Patrick Kelly*1 1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute, University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute, University of Calgary Here we describe a protocol for the automated segmentation of fluorescently labeled tissues on slides using a widefield high-content analysis system (WHCAS). This protocol has wide-ranging applications in any field which involves the quantitation of fluorescent markers in biological tissues including the biological sciences, medical engineering, and health sciences.