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Articles by Volodymyr Tsvilovskyy in JoVE
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays Volodymyr Tsvilovskyy1, Alejandra Solis-Lopez1, Kathrin Öhlenschläger1, Marc Freichel1 1Institute of Pharmacology, Ruprecht-Karls Heidelberg University Here, we present a protocol to isolate and cultivate murine peritoneal mast cells. We also describe two protocols for their functional characterization: a fluorescent imaging of intracellular free Ca2+ concentration and a degranulation assay based on colorimetric quantification of the released β-hexosaminidase.