Wei-Jun Qian Integrative Omics, Biological Sciences Division Pacific Northwest National Laboratory Biography Publications Institution JoVE Articles Wei-Jun Qian has not added a biography. If you are Wei-Jun Qian and would like to personalize this page please email our Author Liaison for assistance. Publications Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample PH ACS Omega. May, 2021 | Pubmed ID: 34056417 Repetitive Diacetyl Vapor Exposure Promotes Ubiquitin Proteasome Stress and Precedes Bronchiolitis Obliterans Pathology Archives of Toxicology. May, 2021 | Pubmed ID: 34031698 Altered β-Cell Prohormone Processing and Secretion in Type 1 Diabetes Diabetes. May, 2021 | Pubmed ID: 33947721 A Comprehensive Urine Proteome Database Generated From Patients With Various Renal Conditions and Prostate Cancer Frontiers in Medicine. 2021 | Pubmed ID: 33928097 Proteomic Profiling of the Substantia Nigra to Identify Determinants of Lewy Body Pathology and Dopaminergic Neuronal Loss Journal of Proteome Research. 05, 2021 | Pubmed ID: 33900085 Enhancing Top-Down Proteomics of Brain Tissue with FAIMS Journal of Proteome Research. 05, 2021 | Pubmed ID: 33856812 Stoichiometric Thiol Redox Proteomics for Quantifying Cellular Responses to Perturbations Antioxidants (Basel, Switzerland). Mar, 2021 | Pubmed ID: 33807006 Defective Insulin Receptor Signaling in HPSCs Skews Pluripotency and Negatively Perturbs Neural Differentiation The Journal of Biological Chemistry. Mar, 2021 | Pubmed ID: 33667549 Surfactant-assisted One-pot Sample Preparation for Label-free Single-cell Proteomics Communications Biology. Mar, 2021 | Pubmed ID: 33649493 Enhancement of Polyhydroxyalkanoate Production by Co-feeding Lignin Derivatives with Glycerol in Pseudomonas Putida KT2440 Biotechnology for Biofuels. Jan, 2021 | Pubmed ID: 33413621 Characterization of Cellular Oxidative Stress Response by Stoichiometric Redox Proteomics American Journal of Physiology. Cell Physiology. 02, 2021 | Pubmed ID: 33264075 Meta-analysis of Peptides to Detect Protein Significance Statistics and Its Interface. 2020 | Pubmed ID: 34055134 Corrigendum: Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney Frontiers in Medicine. 2020 | Pubmed ID: 33511148 Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells Cell Systems. Oct, 2020 | Pubmed ID: 33113355 Protein Thiol Oxidation in the Rat Lung Following E-cigarette Exposure Redox Biology. Oct, 2020 | Pubmed ID: 33080441 Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney Frontiers in Medicine. 2020 | Pubmed ID: 33072769 Stochiometric Quantification of the Thiol Redox Proteome of Macrophages Reveals Subcellular Compartmentalization and Susceptibility to Oxidative Perturbations Redox Biology. Sep, 2020 | Pubmed ID: 32750668 Proteomic Tissue-Based Classifier for Early Prediction of Prostate Cancer Progression Cancers. May, 2020 | Pubmed ID: 32429558 Block Design with Common Reference Samples Enables Robust Large-Scale Label-Free Quantitative Proteome Profiling Journal of Proteome Research. 07, 2020 | Pubmed ID: 32407631 Development of Multiplexed Immuno-N-Terminomics to Reveal the Landscape of Proteolytic Processing in Early Embryogenesis of Analytical Chemistry. 04, 2020 | Pubmed ID: 32196314 Accurate Identification of Deamidation and Citrullination from Global Shotgun Proteomics Data Using a Dual-Search Delta Score Strategy Journal of Proteome Research. 04, 2020 | Pubmed ID: 32175737 Evaluation of the Cytotoxic and Cellular Proteome Impacts of Food-grade TiO (E171) Using Simulated Gastrointestinal Digestions and a Tri-culture Small Intestinal Epithelial Model NanoImpact. Jan, 2020 | Pubmed ID: 32133427 A Proteome-wide Assessment of the Oxidative Stress Paradigm for Metal and Metal-oxide Nanomaterials in Human Macrophages NanoImpact. Jan, 2020 | Pubmed ID: 32133426 Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 As a Target for Type 1 Diabetes Intervention Cell Metabolism. 02, 2020 | Pubmed ID: 31928885 High-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform Analytical Chemistry. 10, 2019 | Pubmed ID: 31509397 Increased β-cell Proliferation Before Immune Cell Invasion Prevents Progression of Type 1 Diabetes Nature Metabolism. May, 2019 | Pubmed ID: 31423480 Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples Analytical Chemistry. 08, 2019 | Pubmed ID: 31241912 The Role of Proteomics in Assessing Beta-cell Dysfunction and Death in Type 1 Diabetes Expert Review of Proteomics. 07, 2019 | Pubmed ID: 31232620 Stable Acinar Progenitor Cell Model Identifies Treacle-Dependent Radioresistance Radiation Research. 08, 2019 | Pubmed ID: 31141469 Response to Comment on Sims Et Al. Proinsulin Secretion Is a Persistent Feature of Type 1 Diabetes. Diabetes Care 2019;42:258-264 Diabetes Care. 05, 2019 | Pubmed ID: 31010952 Architecture and Subunit Arrangement of Native AMPA Receptors Elucidated by Cryo-EM Science (New York, N.Y.). 04, 2019 | Pubmed ID: 30975770 Discovery of Potential Pathways for Biological Conversion of Poplar Wood into Lipids by Co-fermentation of Strains Biotechnology for Biofuels. 2019 | Pubmed ID: 30923568 Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for Comprehensive Quantitative Phosphoproteomic Characterization of Small Populations of Cells Analytical Chemistry. May, 2019 | Pubmed ID: 30843680 Improving Mitochondrial Function with SS-31 Reverses Age-related Redox Stress and Improves Exercise Tolerance in Aged Mice Free Radical Biology & Medicine. 04, 2019 | Pubmed ID: 30597195 Carrier-Assisted Single-Tube Processing Approach for Targeted Proteomics Analysis of Low Numbers of Mammalian Cells Analytical Chemistry. 01, 2019 | Pubmed ID: 30557009 Proinsulin Secretion Is a Persistent Feature of Type 1 Diabetes Diabetes Care. 02, 2019 | Pubmed ID: 30530850 Genomics and Biochemistry Investigation on the Metabolic Pathway of Milled Wood and Alkali Lignin-derived Aromatic Metabolites of SP-35 Biotechnology for Biofuels. 2018 | Pubmed ID: 30603046 Nanoproteomics Comes of Age Expert Review of Proteomics. 11, 2018 | Pubmed ID: 30375896 Insulin Receptor-mediated Signaling Regulates Pluripotency Markers and Lineage Differentiation Molecular Metabolism. 12, 2018 | Pubmed ID: 30316806 Facile Carrier-assisted Targeted Mass Spectrometric Approach for Proteomic Analysis of Low Numbers of Mammalian Cells Communications Biology. 2018 | Pubmed ID: 30271983 Nanowell-mediated Two-dimensional Liquid Chromatography Enables Deep Proteome Profiling of <1000 Mammalian Cells Chemical Science. Sep, 2018 | Pubmed ID: 30210768 Serum Biomarkers for Diagnosis and Prediction of Type 1 Diabetes Translational Research : the Journal of Laboratory and Clinical Medicine. 11, 2018 | Pubmed ID: 30144424 Mass Spectrometry-based Proteomics for System-level Characterization of Biological Responses to Engineered Nanomaterials Analytical and Bioanalytical Chemistry. Sep, 2018 | Pubmed ID: 29947897 Spatially Resolved Proteome Mapping of Laser Capture Microdissected Tissue with Automated Sample Transfer to Nanodroplets Molecular & Cellular Proteomics : MCP. Sep, 2018 | Pubmed ID: 29941660 Fatiguing Contractions Increase Protein S-glutathionylation Occupancy in Mouse Skeletal Muscle Redox Biology. 07, 2018 | Pubmed ID: 29857311 Urinary Virome Perturbations in Kidney Transplantation Frontiers in Medicine. 2018 | Pubmed ID: 29725592 Optimization for Peptide Sample Preparation for Urine Peptidomics Methods in Molecular Biology (Clifton, N.J.). Apr, 2018 | Pubmed ID: 29623538 Targeted Quantification of Phosphorylation Dynamics in the Context of EGFR-MAPK Pathway Analytical Chemistry. 04, 2018 | Pubmed ID: 29584399 Subnanogram Proteomics: Impact of LC Column Selection, MS Instrumentation and Data Analysis Strategy on Proteome Coverage for Trace Samples International Journal of Mass Spectrometry. Apr, 2018 | Pubmed ID: 29576737 Nanodroplet Processing Platform for Deep and Quantitative Proteome Profiling of 10-100 Mammalian Cells Nature Communications. 02, 2018 | Pubmed ID: 29491378 Multiplexed Targeted Mass Spectrometry Assays for Prostate Cancer-associated Urinary Proteins Oncotarget. Nov, 2017 | Pubmed ID: 29254211 LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers Methods in Molecular Biology (Clifton, N.J.). Nov, 2017 | Pubmed ID: 29116567 -Nitrosylation Inhibits the Kinase Activity of Tomato Phosphoinositide-dependent Kinase 1 (PDK1) The Journal of Biological Chemistry. 12, 2017 | Pubmed ID: 28972151 Functional Enzyme-Based Approach for Linking Microbial Community Functions with Biogeochemical Process Kinetics Environmental Science & Technology. Oct, 2017 | Pubmed ID: 28891285 Quantification of Mutant SPOP Proteins in Prostate Cancer Using Mass Spectrometry-based Targeted Proteomics Journal of Translational Medicine. Aug, 2017 | Pubmed ID: 28810879 Advances in Microscale Separations Towards Nanoproteomics Applications Journal of Chromatography. A. Nov, 2017 | Pubmed ID: 28765000 Deep-Dive Targeted Quantification for Ultrasensitive Analysis of Proteins in Nondepleted Human Blood Plasma/Serum and Tissues Analytical Chemistry. Sep, 2017 | Pubmed ID: 28724286 Targeted Proteomic Assays for Quantitation of Proteins Identified by Proteogenomic Analysis of Ovarian Cancer Scientific Data. 07, 2017 | Pubmed ID: 28722704 Quantitative Proteomics Identifies Altered O-GlcNAcylation of Structural, Synaptic and Memory-associated Proteins in Alzheimer's Disease The Journal of Pathology. Sep, 2017 | Pubmed ID: 28657654 Targeted Quantification of Functional Enzyme Dynamics in Environmental Samples for Microbially Mediated Biogeochemical Processes Environmental Microbiology Reports. Oct, 2017 | Pubmed ID: 28618201 Quantitative Proteomic Characterization of Redox-dependent Post-translational Modifications on Protein Cysteines Molecular BioSystems. May, 2017 | Pubmed ID: 28357434 Biodegradation of Alkaline Lignin by L1 Biotechnology for Biofuels. 2017 | Pubmed ID: 28239416 An Efficient Method for Native Protein Purification in the Selected Range from Prostate Cancer Tissue Digests Chinese Clinical Oncology. Dec, 2016 | Pubmed ID: 28061542 Conservation of Protein Abundance Patterns Reveals the Regulatory Architecture of the EGFR-MAPK Pathway Science Signaling. 07, 2016 | Pubmed ID: 27405981 Advances in Targeted Proteomics and Applications to Biomedical Research Proteomics. 08, 2016 | Pubmed ID: 27302376 Mining the Human Urine Proteome for Monitoring Renal Transplant Injury Kidney International. 06, 2016 | Pubmed ID: 27165815 The Current State of the Art of Quantitative Phosphoproteomics and Its Applications to Diabetes Research Expert Review of Proteomics. 2016 | Pubmed ID: 26960075 9-Fluorenylmethyl (Fm) Disulfides: Biomimetic Precursors for Persulfides Organic Letters. Mar, 2016 | Pubmed ID: 26870874 Contributions of Immunoaffinity Chromatography to Deep Proteome Profiling of Human Biofluids Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. May, 2016 | Pubmed ID: 26868616 Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays Methods in Molecular Biology (Clifton, N.J.). 2016 | Pubmed ID: 26867747 SNaPP: Simplified Nanoproteomics Platform for Reproducible Global Proteomic Analysis of Nanogram Protein Quantities Endocrinology. Mar, 2016 | Pubmed ID: 26745641 Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays Clinical Chemistry. Jan, 2016 | Pubmed ID: 26719571 SerpinB1 Promotes Pancreatic β Cell Proliferation Cell Metabolism. Jan, 2016 | Pubmed ID: 26701651 Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function During Nanoparticle-Induced Oxidative Stress ACS Nano. Jan, 2016 | Pubmed ID: 26700264 The Clinical Impact of Recent Advances in LC-MS for Cancer Biomarker Discovery and Verification Expert Review of Proteomics. 2016 | Pubmed ID: 26581546 The Measurement of Reversible Redox Dependent Post-translational Modifications and Their Regulation of Mitochondrial and Skeletal Muscle Function Frontiers in Physiology. 2015 | Pubmed ID: 26635632 Preserved DNA Damage Checkpoint Pathway Protects Against Complications in Long-Standing Type 1 Diabetes Cell Metabolism. Aug, 2015 | Pubmed ID: 26244933 Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics Journal of Proteome Research. Aug, 2015 | Pubmed ID: 26151086 Analytical Platform Evaluation for Quantification of ERG in Prostate Cancer Using Protein and MRNA Detection Methods Journal of Translational Medicine. Feb, 2015 | Pubmed ID: 25889691 Sensitive Targeted Quantification of ERK Phosphorylation Dynamics and Stoichiometry in Human Cells Without Affinity Enrichment Analytical Chemistry. Jan, 2015 | Pubmed ID: 25517423 The Identification of Novel Potential Injury Mechanisms and Candidate Biomarkers in Renal Allograft Rejection by Quantitative Proteomics Molecular & Cellular Proteomics : MCP. Feb, 2014 | Pubmed ID: 33498143 Perturbations in the Urinary Exosome in Transplant Rejection Frontiers in Medicine. 2014 | Pubmed ID: 25593928 Proteomic Approaches for Site-specific O-GlcNAcylation Analysis Bioanalysis. 2014 | Pubmed ID: 25411699 Brain Insulin Lowers Circulating BCAA Levels by Inducing Hepatic BCAA Catabolism Cell Metabolism. Nov, 2014 | Pubmed ID: 25307860 Antibody-independent Targeted Quantification of TMPRSS2-ERG Fusion Protein Products in Prostate Cancer Molecular Oncology. Oct, 2014 | Pubmed ID: 25266362 Expediting SRM Assay Development for Large-scale Targeted Proteomics Experiments Journal of Proteome Research. Oct, 2014 | Pubmed ID: 25145539 A Method to Determine Lysine Acetylation Stoichiometries International Journal of Proteomics. 2014 | Pubmed ID: 25143833 Proteome-wide Light/dark Modulation of Thiol Oxidation in Cyanobacteria Revealed by Quantitative Site-specific Redox Proteomics Molecular & Cellular Proteomics : MCP. Dec, 2014 | Pubmed ID: 25118246 Detecting Differential Protein Expression in Large-scale Population Proteomics Bioinformatics (Oxford, England). Oct, 2014 | Pubmed ID: 24928210 High and Low Doses of Ionizing Radiation Induce Different Secretome Profiles in a Human Skin Model PloS One. 2014 | Pubmed ID: 24642900 Optimization for Peptide Sample Preparation for Urine Peptidomics Clinical Proteomics. Feb, 2014 | Pubmed ID: 24568099 Resin-assisted Enrichment of Thiols As a General Strategy for Proteomic Profiling of Cysteine-based Reversible Modifications Nature Protocols. Jan, 2014 | Pubmed ID: 24336471 The Identification of Novel Potential Injury Mechanisms and Candidate Biomarkers in Renal Allograft Rejection by Quantitative Proteomics Molecular & Cellular Proteomics : MCP. Feb, 2014 | Pubmed ID: 24335474 Proteomic Identification and Quantification of S-glutathionylation in Mouse Macrophages Using Resin-assisted Enrichment and Isobaric Labeling Free Radical Biology & Medicine. Feb, 2014 | Pubmed ID: 24333276 A Highly Sensitive Targeted Mass Spectrometric Assay for Quantification of AGR2 Protein in Human Urine and Serum Journal of Proteome Research. Feb, 2014 | Pubmed ID: 24251762 Impact of Alg3 Gene Deletion on Growth, Development, Pigment Production, Protein Secretion, and Functions of Recombinant Trichoderma Reesei Cellobiohydrolases in Aspergillus Niger Fungal Genetics and Biology : FG & B. Dec, 2013 | Pubmed ID: 24076077 Long-gradient Separations Coupled with Selected Reaction Monitoring for Highly Sensitive, Large Scale Targeted Protein Quantification in a Single Analysis Analytical Chemistry. Oct, 2013 | Pubmed ID: 24004026 Resin-assisted Enrichment of N-terminal Peptides for Characterizing Proteolytic Processing Analytical Chemistry. Jul, 2013 | Pubmed ID: 23772796 Targeted Quantification of Low Ng/mL Level Proteins in Human Serum Without Immunoaffinity Depletion Journal of Proteome Research. Jul, 2013 | Pubmed ID: 23763644 Evaluation of Selected Binding Domains for the Analysis of Ubiquitinated Proteomes Journal of the American Society for Mass Spectrometry. Aug, 2013 | Pubmed ID: 23649778 Trauma-associated Human Neutrophil Alterations Revealed by Comparative Proteomics Profiling Proteomics. Clinical Applications. Aug, 2013 | Pubmed ID: 23589343 Determination of Burn Patient Outcome by Large-scale Quantitative Discovery Proteomics Critical Care Medicine. Jun, 2013 | Pubmed ID: 23507713 Synthesis and Application of an Environmentally Insensitive Cy3-based Arsenical Fluorescent Probe to Identify Adaptive Microbial Responses Involving Proximal Dithiol Oxidation Journal of the American Chemical Society. Mar, 2013 | Pubmed ID: 23379730 Liver-Derived Systemic Factors Drive β Cell Hyperplasia in Insulin-Resistant States Cell Reports. Jan, 2013 | Pubmed ID: 23375376 In-source Fragmentation and the Sources of Partially Tryptic Peptides in Shotgun Proteomics Journal of Proteome Research. Feb, 2013 | Pubmed ID: 23268687 Quantitative Site-specific Reactivity Profiling of S-nitrosylation in Mouse Skeletal Muscle Using Cysteinyl Peptide Enrichment Coupled with Mass Spectrometry Free Radical Biology & Medicine. Dec, 2012 | Pubmed ID: 23277143 Antibody-free, Targeted Mass-spectrometric Approach for Quantification of Proteins at Low Picogram Per Milliliter Levels in Human Plasma/serum Proceedings of the National Academy of Sciences of the United States of America. Sep, 2012 | Pubmed ID: 22949669 Analysis of Serum Total and Free PSA Using Immunoaffinity Depletion Coupled to SRM: Correlation with Clinical Immunoassay Tests Journal of Proteomics. Aug, 2012 | Pubmed ID: 22846433 Advancing the Sensitivity of Selected Reaction Monitoring-based Targeted Quantitative Proteomics Proteomics. Apr, 2012 | Pubmed ID: 22577010 Identification of Phosphorylated Butyrylcholinesterase in Human Plasma Using Immunoaffinity Purification and Mass Spectrometry Analytica Chimica Acta. Apr, 2012 | Pubmed ID: 22444575 Simple Sodium Dodecyl Sulfate-assisted Sample Preparation Method for LC-MS-based Proteomics Applications Analytical Chemistry. Mar, 2012 | Pubmed ID: 22339560 Mapping N-linked Glycosylation Sites in the Secretome and Whole Cells of Aspergillus Niger Using Hydrazide Chemistry and Mass Spectrometry Journal of Proteome Research. Jan, 2012 | Pubmed ID: 22136231 IgY14 and SuperMix Immunoaffinity Separations Coupled with Liquid Chromatography-mass Spectrometry for Human Plasma Proteomics Biomarker Discovery Methods (San Diego, Calif.). Feb, 2012 | Pubmed ID: 21925605 18O-labeled Proteome Reference As Global Internal Standards for Targeted Quantification by Selected Reaction Monitoring-mass Spectrometry Molecular & Cellular Proteomics : MCP. Dec, 2011 | Pubmed ID: 21988777 Quantitative Proteomics Analysis of Adsorbed Plasma Proteins Classifies Nanoparticles with Different Surface Properties and Size Proteomics. Dec, 2011 | Pubmed ID: 21956884 Unraveling Pancreatic Islet Biology by Quantitative Proteomics Expert Review of Proteomics. Aug, 2011 | Pubmed ID: 21819304 Identification of C-type Heme-containing Peptides Using Nonactivated Immobilized Metal Affinity Chromatography Resin Enrichment and Higher-energy Collisional Dissociation Analytical Chemistry. Oct, 2011 | Pubmed ID: 21740036 Liquid Chromatography-mass Spectrometry-based Quantitative Proteomics The Journal of Biological Chemistry. Jul, 2011 | Pubmed ID: 21632532 Trypsin-catalyzed Oxygen-18 Labeling for Quantitative Proteomics Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21604114 Enhanced Sensitivity for Selected Reaction Monitoring Mass Spectrometry-based Targeted Proteomics Using a Dual Stage Electrodynamic Ion Funnel Interface Molecular & Cellular Proteomics : MCP. Feb, 2011 | Pubmed ID: 20410378 Improved LC-MS/MS Spectral Counting Statistics by Recovering Low-scoring Spectra Matched to Confidently Identified Peptide Sequences Journal of Proteome Research. Nov, 2010 | Pubmed ID: 20812748 Clinical Microfluidics for Neutrophil Genomics and Proteomics Nature Medicine. Sep, 2010 | Pubmed ID: 20802500 Plasma Proteome Response to Severe Burn Injury Revealed by 18O-labeled "universal" Reference-based Quantitative Proteomics Journal of Proteome Research. Sep, 2010 | Pubmed ID: 20698492 CD90/THY1 is Overexpressed in Prostate Cancer-associated Fibroblasts and Could Serve As a Cancer Biomarker Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc. Oct, 2010 | Pubmed ID: 20562849 Shotgun Proteomics Identifies Proteins Specific for Acute Renal Transplant Rejection Proteomics. Clinical Applications. Jan, 2010 | Pubmed ID: 20543976 Sensitive Immunoassays of Nitrated Fibrinogen in Human Biofluids Talanta. Jun, 2010 | Pubmed ID: 20441955 Quantitative Analysis of Cell Surface Membrane Proteins Using Membrane-impermeable Chemical Probe Coupled with 18O Labeling Journal of Proteome Research. May, 2010 | Pubmed ID: 20380418 Region-specific Protein Abundance Changes in the Brain of MPTP-induced Parkinson's Disease Mouse Model Journal of Proteome Research. Mar, 2010 | Pubmed ID: 20155936 Endogenous 3,4-dihydroxyphenylalanine and Dopaquinone Modifications on Protein Tyrosine: Links to Mitochondrially Derived Oxidative Stress Via Hydroxyl Radical Molecular & Cellular Proteomics : MCP. Jun, 2010 | Pubmed ID: 20124354 Mapping Protein Abundance Patterns in the Brain Using Voxelation Combined with Liquid Chromatography and Mass Spectrometry Methods (San Diego, Calif.). Feb, 2010 | Pubmed ID: 19654045 N-terminal Enrichment: Developing a Protocol to Detect Specific Proteolytic Fragments Journal of Biomolecular Techniques : JBT. Dec, 2009 | Pubmed ID: 19949699 Mouse-specific Tandem IgY7-SuperMix Immunoaffinity Separations for Improved LC-MS/MS Coverage of the Plasma Proteome Journal of Proteome Research. Nov, 2009 | Pubmed ID: 19722698 A Statistical Framework for Protein Quantitation in Bottom-up MS-based Proteomics Bioinformatics (Oxford, England). Aug, 2009 | Pubmed ID: 19535538 An Extensive Survey of Tyrosine Phosphorylation Revealing New Sites in Human Mammary Epithelial Cells Journal of Proteome Research. Aug, 2009 | Pubmed ID: 19534553 Dual Spatial Maps of Transcript and Protein Abundance in the Mouse Brain Expert Review of Proteomics. Jun, 2009 | Pubmed ID: 19489697 A Simple Procedure for Effective Quenching of Trypsin Activity and Prevention of 18O-labeling Back-exchange Journal of Proteome Research. May, 2009 | Pubmed ID: 19222237 Large-scale Multiplexed Quantitative Discovery Proteomics Enabled by the Use of an (18)O-labeled "universal" Reference Sample Journal of Proteome Research. Jan, 2009 | Pubmed ID: 19053531 Quantitative Phosphoproteome Analysis of Lysophosphatidic Acid Induced Chemotaxis Applying Dual-step (18)O Labeling Coupled with Immobilized Metal-ion Affinity Chromatography Journal of Proteome Research. Oct, 2008 | Pubmed ID: 18785766 Enhanced Detection of Low Abundance Human Plasma Proteins Using a Tandem IgY12-SuperMix Immunoaffinity Separation Strategy Molecular & Cellular Proteomics : MCP. Oct, 2008 | Pubmed ID: 18632595 Characterization of the Mouse Pancreatic Islet Proteome and Comparative Analysis with Other Mouse Tissues Journal of Proteome Research. Aug, 2008 | Pubmed ID: 18570455 DAnTE: a Statistical Tool for Quantitative Analysis of -omics Data Bioinformatics (Oxford, England). Jul, 2008 | Pubmed ID: 18453552 Quantitative Proteome Mapping of Nitrotyrosines Methods in Enzymology. 2008 | Pubmed ID: 18423218 Proteome-wide Identification of Proteins and Their Modifications with Decreased Ambiguities and Improved False Discovery Rates Using Unique Sequence Tags Analytical Chemistry. Mar, 2008 | Pubmed ID: 18271604 Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis Revealed by Proteomic and Transcriptomic Analyses of the Striata in Two Mouse Models of Parkinson's Disease Journal of Proteome Research. Feb, 2008 | Pubmed ID: 18173235 Elimination of Systematic Mass Measurement Errors in Liquid Chromatography-mass Spectrometry Based Proteomics Using Regression Models and a Priori Partial Knowledge of the Sample Content Analytical Chemistry. Feb, 2008 | Pubmed ID: 18163597 Application of Proteomics in the Discovery of Candidate Protein Biomarkers in a Diabetes Autoantibody Standardization Program Sample Subset Journal of Proteome Research. Feb, 2008 | Pubmed ID: 18092746 Capillary-based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-phase LC-ESI-MS Analytical Chemistry. Jan, 2008 | Pubmed ID: 18044958 Accurate Mass Measurements in Proteomics Chemical Reviews. Aug, 2007 | Pubmed ID: 17649984 A Genome-scale Map of Expression for a Mouse Brain Section Obtained Using Voxelation Physiological Genomics. Aug, 2007 | Pubmed ID: 17504947 A Method for Selective Enrichment and Analysis of Nitrotyrosine-containing Peptides in Complex Proteome Samples Journal of Proteome Research. Jun, 2007 | Pubmed ID: 17497906 Profiling Signaling Polarity in Chemotactic Cells Proceedings of the National Academy of Sciences of the United States of America. May, 2007 | Pubmed ID: 17494752 Quantitative Analysis of Human Immunodeficiency Virus Type 1-infected CD4+ Cell Proteome: Dysregulated Cell Cycle Progression and Nuclear Transport Coincide with Robust Virus Production Journal of Virology. Jul, 2007 | Pubmed ID: 17494070 The Use of a Quantitative Cysteinyl-peptide Enrichment Technology for High-throughput Quantitative Proteomics Methods in Molecular Biology (Clifton, N.J.). 2007 | Pubmed ID: 17484113 Quantitative Proteomic Approaches for Studying Phosphotyrosine Signaling Expert Review of Proteomics. Feb, 2007 | Pubmed ID: 17288512 Spatial Mapping of Protein Abundances in the Mouse Brain by Voxelation Integrated with High-throughput Liquid Chromatography-mass Spectrometry Genome Research. Mar, 2007 | Pubmed ID: 17255552 Characterization of the Human Pancreatic Islet Proteome by Two-dimensional LC/MS/MS Journal of Proteome Research. Dec, 2006 | Pubmed ID: 17137336 Advances and Challenges in Liquid Chromatography-mass Spectrometry-based Proteomics Profiling for Clinical Applications Molecular & Cellular Proteomics : MCP. Oct, 2006 | Pubmed ID: 16887931 Evaluation of Multiprotein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry Molecular & Cellular Proteomics : MCP. Nov, 2006 | Pubmed ID: 16854842 Endogenously Nitrated Proteins in Mouse Brain: Links to Neurodegenerative Disease Biochemistry. Jul, 2006 | Pubmed ID: 16800626 High Dynamic Range Characterization of the Trauma Patient Plasma Proteome Molecular & Cellular Proteomics : MCP. Oct, 2006 | Pubmed ID: 16684767 Characterization of the Mouse Brain Proteome Using Global Proteomic Analysis Complemented with Cysteinyl-peptide Enrichment Journal of Proteome Research. Feb, 2006 | Pubmed ID: 16457602 Normalization Approaches for Removing Systematic Biases Associated with Mass Spectrometry and Label-free Proteomics Journal of Proteome Research. Feb, 2006 | Pubmed ID: 16457593 Advances in Proteomics Data Analysis and Display Using an Accurate Mass and Time Tag Approach Mass Spectrometry Reviews. May-Jun, 2006 | Pubmed ID: 16429408 Development and Evaluation of a Micro- and Nanoscale Proteomic Sample Preparation Method Journal of Proteome Research. Nov-Dec, 2005 | Pubmed ID: 16335993 Human Plasma N-glycoproteome Analysis by Immunoaffinity Subtraction, Hydrazide Chemistry, and Mass Spectrometry Journal of Proteome Research. Nov-Dec, 2005 | Pubmed ID: 16335952 Utilizing Human Blood Plasma for Proteomic Biomarker Discovery Journal of Proteome Research. Jul-Aug, 2005 | Pubmed ID: 16083256 Quantitative Proteome Analysis of Human Plasma Following in Vivo Lipopolysaccharide Administration Using 16O/18O Labeling and the Accurate Mass and Time Tag Approach Molecular & Cellular Proteomics : MCP. May, 2005 | Pubmed ID: 15753121 Improved Proteome Coverage by Using High Efficiency Cysteinyl Peptide Enrichment: the Human Mammary Epithelial Cell Proteome Proteomics. Apr, 2005 | Pubmed ID: 15742320 Probability-based Evaluation of Peptide and Protein Identifications from Tandem Mass Spectrometry and SEQUEST Analysis: the Human Proteome Journal of Proteome Research. Jan-Feb, 2005 | Pubmed ID: 15707357 Targeted Comparative Proteomics by Liquid Chromatography-tandem Fourier Ion Cyclotron Resonance Mass Spectrometry Analytical Chemistry. Jan, 2005 | Pubmed ID: 15649034 Comparative Proteome Analyses of Human Plasma Following in Vivo Lipopolysaccharide Administration Using Multidimensional Separations Coupled with Tandem Mass Spectrometry Proteomics. Feb, 2005 | Pubmed ID: 15627965 High-throughput Proteomics Using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Expert Review of Proteomics. Jun, 2004 | Pubmed ID: 15966802 Simultaneous Monitoring of Zn2+ Secretion and Intracellular Ca2+ from Islets and Islet Cells by Fluorescence Microscopy BioTechniques. Dec, 2004 | Pubmed ID: 15597541 High-throughput Comparative Proteome Analysis Using a Quantitative Cysteinyl-peptide Enrichment Technology Analytical Chemistry. Sep, 2004 | Pubmed ID: 15362891 Phosphoprotein Isotope-coded Solid-phase Tag Approach for Enrichment and Quantitative Analysis of Phosphopeptides from Complex Mixtures Analytical Chemistry. Oct, 2003 | Pubmed ID: 14714534 Late-life Restoration of Mitochondrial Function Reverses Cardiac Dysfunction in Old Mice ELife. | Pubmed ID: 32648542 Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation Matthew J. Gaffrey1, Nicholas J. Day1, Xiaolu Li2, Wei-Jun Qian1 1Integrative Omics, Biological Sciences Division, Pacific Northwest National Laboratory, 2Bioproducts Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University JoVE 62671 Biochemistry Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells Kendall Martin1, Tong Zhang1, Pengfei Zhang2, William B. Chrisler1, Fillmore L. Thomas3, Fen Liu4, Tao Liu1, Wei-Jun Qian1, Richard D. Smith1, Tujin Shi1 1Biological Sciences Division, Pacific Northwest National Laboratory, 2NHC Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, 3Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 4Department of Critical Care Medicine, The First Affiliated Hospital of Nanchang University JoVE 61797 Biochemistry
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation Matthew J. Gaffrey1, Nicholas J. Day1, Xiaolu Li2, Wei-Jun Qian1 1Integrative Omics, Biological Sciences Division, Pacific Northwest National Laboratory, 2Bioproducts Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University JoVE 62671 Biochemistry
Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells Kendall Martin1, Tong Zhang1, Pengfei Zhang2, William B. Chrisler1, Fillmore L. Thomas3, Fen Liu4, Tao Liu1, Wei-Jun Qian1, Richard D. Smith1, Tujin Shi1 1Biological Sciences Division, Pacific Northwest National Laboratory, 2NHC Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, 3Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 4Department of Critical Care Medicine, The First Affiliated Hospital of Nanchang University JoVE 61797 Biochemistry
