In JoVE (4)
- Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
- Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
- Dissection of Larval Zebrafish Gonadal Tissue
- In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
Articles by Wei Chen in JoVE
Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell Yunfeng Chen*1, Baoyu Liu*2, Lining Ju*3, Jinsung Hong*1, Qinghua Ji4,5, Wei Chen6, Cheng Zhu1 1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University We describe a technique for concurrently measuring force-regulated single receptor-ligand binding kinetics and real-time imaging of calcium signaling in a single T lymphocyte.
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors Wei Wen1, Jian-Ping Zhang1, Wanqiu Chen2, Cameron Arakaki2, Xiaolan Li1,2, David Baylink2, Gary D. Botimer3, Jing Xu1, Weiping Yuan1, Tao Cheng1,4,5,6,7, Xiao-Bing Zhang1,2 1State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 2Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 3Department of Orthopaedic Surgery, Loma Linda University, 4Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, 5Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, 6Collaborative Innovation Center for Cancer Medicine, 7Tianjin Key Laboratory of Blood Cell Therapy and Technology This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.
Dissection of Larval Zebrafish Gonadal Tissue Xinjian Wang*1, Sijie Chen*1, Wei Zhang1, Yiyuan Ren1, Quan Zhang1, Gang Peng1 1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity Shen Zhang1, Yonggen Wu1, Yimin Weng2, Zhihui Xu1, Wenmin Chen3, Dahan Zheng4, Wei Lin5, Jun Liu6, Ying Zhou1,7 1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
Other articles by Wei Chen on PubMed
Evaluation of Alginate Coated Chitosan Membrane for Guided Tissue Regeneration Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference. 2005 | Pubmed ID: 17281335 Chitosan membranes prepared by a thermal induced phase separation method and then alginate was coated onto the membrane on one side by a modified dialysis apparatus to prepare alginate/chitosan membranes (A/C membranes). ESCA analysis, SEM observation and contact angle measurements were conducted to evaluate the differences existed in the surface properties. Besides, mechanical strength, degradation behavior and especially, cell adhesion test was also examined to survey the feasibility of using this type of A/C membrane in guided tissue regeneration (GTR) application. The preliminary results obtained revealed that alginate could effectively be coated onto the chitosan membrane (from ESCA and SEM results). The contact angle decreased on the alginate side as compared with the chitosan-side (from 88.4deg to 34.2deg). The A/C membrane had a water higher content of 71.8 % as compare with the chitosan membrane (61.8%). Chitosan membranes coated with alginate also increased the Young's modulus and the ultimate strength, whereas, the elongation of these membranes were remained at the same range. A/C membrane and chitosan membrane prepared in this study degraded to about 75% of initial weight after a 30-day in vitro shaking test. The 3T3 fibroblast cells showed less adhesive on alginate side as compared with the chitosan side and glass surface in cell adhesion test. The obtained results from this study suggested that the alginate molecules could be coated onto the chitosan membrane by our modified dialysis apparatus and provided a different surface morphological structures and properties of chitosan membrane. This A/C membrane could provide extra benefits and potentials than chitosan membrane used in GTR applications.
Efficacy and Safety of Nebivolol in Hispanics with Stage I-II Hypertension: a Randomized Placebo-controlled Trial Therapeutic Advances in Cardiovascular Disease. Dec, 2010 | Pubmed ID: 21088095 Hispanics have lower rates of hypertension control compared with black and white patients. Nebivolol is a vasodilatory β1-selective blocker, with neutral metabolic effects. This phase IV trial evaluated the efficacy and safety of nebivolol in Hispanics with stage I-II hypertension.