Articles by Weitong Chen in JoVE

• Bioengineering

  
  Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy Lijuan He*^1,2, Alexandra Sneider*¹, Weitong Chen¹, Michelle Karl¹, Vishnu Prasath³, Pei-Hsun Wu^1,2, Gunnar Mattson³, Denis Wirtz^{1,2,4 1}Department of Chemical and Biomolecular Engineering, Johns Hopkins University, ²Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, ³Department of Biomedical Engineering, Johns Hopkins University, ⁴Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine This protocol efficiently studies mammalian cell division in 3D collagen matrices by integrating synchronization of cell division, monitoring of division events in 3D matrices using live-cell imaging technique, time-resolved confocal reflection microscopy and quantitative imaging analysis.