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Articles by William A Petri, Jr in JoVE
Development of a Negative Selectable Marker for Entamoeba histolytica Mayuresh M Abhyankar1, Sarah M Haviland1, Carol A Gilchrist1, William A Petri, Jr.1 1Division of Infectious Disease and International Health, University of Virginia Health System We report development of a negative selection system in E. histolytica based upon transgenic expression of a chimeric protein (FCU1) and selection with the prodrug 5-fluorocytosine. The FCU1 protein is a fusion of yeast cytosine deaminase and uracil phosphoribosyltransferase. Expression of FCU1 resulted in increased E. histolytica sensitivity towards 5-fluorocytosine.