In JoVE (1)
Other Publications (1)
Articles by William Pau in JoVE
Remotely Supervised Transcranial Direct Current Stimulation: An Update on Safety and Tolerability Michael T. Shaw1, Margaret Kasschau2, Bryan Dobbs1, Natalie Pawlak1, William Pau1, Kathleen Sherman1, Marom Bikson3, Abhishek Datta4, Leigh E. Charvet1 1New York University, Langone Medical Center, 2Stony Brook Medicine, 3City College of New York, 4Soterix Medical This manuscript provides an updated remote supervision protocol that enables participation in transcranial direct current stimulation (tDCS) clinical trials while receiving treatment sessions from home. The protocol has been successfully piloted in both patients with multiple sclerosis and Parkinson's disease.
Other articles by William Pau on PubMed
Characterization of a High-affinity Sialic Acid-specific CBM40 from Clostridium Perfringens and Engineering of a Divalent Form The Biochemical Journal. Jul, 2016 | Pubmed ID: 27208171 CBMs (carbohydrate-binding modules) are a class of polypeptides usually associated with carbohydrate-active enzymatic sites. We have characterized a new member of the CBM40 family, coded from a section of the gene NanI from Clostridium perfringens Glycan arrays revealed its preference towards α(2,3)-linked sialosides, which was confirmed and quantified by calorimetric studies. The CBM40 binds to α(2,3)-sialyl-lactose with a Kd of ∼30 μM, the highest affinity value for this class of proteins. Inspired by lectins' structure and their arrangement as multimeric proteins, we have engineered a dimeric form of the CBM, and using SPR (surface plasmon resonance) we have observed 6-11-fold binding increases due to the avidity affect. The structures of the CBM, resolved by X-ray crystallography, in complex with α(2,3)- or α(2,6)-sialyl-lactose explain its binding specificity and unusually strong binding.