Wim Hagen Structural & Computational Biology unit Heidelberg EMBL Biography Publications Institution JoVE Articles Wim Hagen Weapon systems engineer in the Royal Netherlands Navy (10 years), various roles (production, customer service, R&D, application support & management) at FEI Company (currently Thermo Fisher Scientific) in The Netherlands and the United States of America (ten years), responsible for cryo-EM operations at EMBL Heidelberg Germany since 2011. Publications Defocus-dependent Thon-ring Fading Ultramicroscopy. Mar, 2021 | Pubmed ID: 33524638 Structural Impact of K63 Ubiquitin on Yeast Translocating Ribosomes Under Oxidative Stress Proceedings of the National Academy of Sciences of the United States of America. 09, 2020 | Pubmed ID: 32855298 In Situ Structural Analysis of SARS-CoV-2 Spike Reveals Flexibility Mediated by Three Hinges Science (New York, N.Y.). 10, 2020 | Pubmed ID: 32817270 Combining High Throughput and High Quality for Cryo-electron Microscopy Data Collection Acta Crystallographica. Section D, Structural Biology. Aug, 2020 | Pubmed ID: 32744254 In-cell Architecture of an Actively Transcribing-translating Expressome Science (New York, N.Y.). 07, 2020 | Pubmed ID: 32732422 TEM Bright Field Imaging of Thick Specimens: Nodes in Thon Ring Patterns Ultramicroscopy. 09, 2020 | Pubmed ID: 32559707 Structural Basis for RNA Polymerase III Transcription Repression by Maf1 Nature Structural & Molecular Biology. 03, 2020 | Pubmed ID: 32066962 Benchmarking Tomographic Acquisition Schemes for High-resolution Structural Biology Nature Communications. 02, 2020 | Pubmed ID: 32054835 Structural Basis of P62/SQSTM1 Helical Filaments and Their Role in Cellular Cargo Uptake Nature Communications. 01, 2020 | Pubmed ID: 31974402 Molecular Mechanism of Promoter Opening by RNA Polymerase III Nature. 01, 2018 | Pubmed ID: 29345638 9Å Structure of the COPI Coat Reveals That the Arf1 GTPase Occupies Two Contrasting Molecular Environments ELife. 06, 2017 | Pubmed ID: 28621666 Structure of the Hexagonal Surface Layer on Caulobacter Crescentus Cells Nature Microbiology. Apr, 2017 | Pubmed ID: 28418382 Implementation of a Cryo-electron Tomography Tilt-scheme Optimized for High Resolution Subtomogram Averaging Journal of Structural Biology. 02, 2017 | Pubmed ID: 27313000 The Structure and Flexibility of Conical HIV-1 Capsids Determined Within Intact Virions Science (New York, N.Y.). 12, 2016 | Pubmed ID: 27980210 Molecular Structures of Transcribing RNA Polymerase I Molecular Cell. 12, 2016 | Pubmed ID: 27867008 An Atomic Model of HIV-1 Capsid-SP1 Reveals Structures Regulating Assembly and Maturation Science (New York, N.Y.). Jul, 2016 | Pubmed ID: 27417497 Molecular Architecture of the Inner Ring Scaffold of the Human Nuclear Pore Complex Science (New York, N.Y.). Apr, 2016 | Pubmed ID: 27081072 Molecular Structures of Unbound and Transcribing RNA Polymerase III Nature. Dec, 2015 | Pubmed ID: 26605533 The Structure of Immature Virus-Like Rous Sarcoma Virus Gag Particles Reveals a Structural Role for the P10 Domain in Assembly Journal of Virology. Oct, 2015 | Pubmed ID: 26223638 The Selective Autophagy Receptor P62 Forms a Flexible Filamentous Helical Scaffold Cell Reports. May, 2015 | Pubmed ID: 25921531 An Organized Co-assembly of Clathrin Adaptors is Essential for Endocytosis Developmental Cell. Apr, 2015 | Pubmed ID: 25898165 Seeing Tobacco Mosaic Virus Through Direct Electron Detectors Journal of Structural Biology. Feb, 2015 | Pubmed ID: 25528571 Structure of the Immature HIV-1 Capsid in Intact Virus Particles at 8.8 Å Resolution Nature. Jan, 2015 | Pubmed ID: 25363765 Cryo-electron Microscopy of Tubular Arrays of HIV-1 Gag Resolves Structures Essential for Immature Virus Assembly Proceedings of the National Academy of Sciences of the United States of America. Jun, 2014 | Pubmed ID: 24843179 SNARE and Regulatory Proteins Induce Local Membrane Protrusions to Prime Docked Vesicles for Fast Calcium-triggered Fusion EMBO Reports. Mar, 2014 | Pubmed ID: 24493260 Determination of Protein Structure at 8.5Å Resolution Using Cryo-electron Tomography and Sub-tomogram Averaging Journal of Structural Biology. Dec, 2013 | Pubmed ID: 24184468 Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition Felix Weis1, Wim J. H. Hagen1, Martin Schorb2, Simone Mattei1,3 1Structural and Computational Biology Unit, European Molecular Biology Laboratory, 2Electron Microscopy Core Facility, European Molecular Biology Laboratory, 3Imaging Centre, European Molecular Biology Laboratory JoVE 62383 Biology
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition Felix Weis1, Wim J. H. Hagen1, Martin Schorb2, Simone Mattei1,3 1Structural and Computational Biology Unit, European Molecular Biology Laboratory, 2Electron Microscopy Core Facility, European Molecular Biology Laboratory, 3Imaging Centre, European Molecular Biology Laboratory JoVE 62383 Biology
