In JoVE (1)
Other Publications (1)
Articles by Wycliffe O. Masanta in JoVE
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP) Andreas E. Zautner1, Raimond Lugert1, Wycliffe O. Masanta1, Michael Weig1, Uwe Groß1, Oliver Bader1 1Institut für Medizinische Mikrobiologie, Universitätsmedizin Göttingen Mass spectrometry-based phyloproteomics (MSPP) was used to type a collection of Campylobacter jejuni ssp. doylei isolates at the strain level in comparison to multilocus sequence typing (MLST).
Other articles by Wycliffe O. Masanta on PubMed
Artificially Designed Pathogens - a Diagnostic Option for Future Military Deployments Military Medical Research. 2015 | Pubmed ID: 26157585 Diagnostic microbial isolates of bio-safety levels 3 and 4 are difficult to handle in medical field camps under military deployment settings. International transport of such isolates is challenging due to restrictions by the International Air Transport Association. An alternative option might be inactivation and sequencing of the pathogen at the deployment site with subsequent sequence-based revitalization in well-equipped laboratories in the home country for further scientific assessment. A literature review was written based on a PubMed search. First described for poliovirus in 2002, de novo synthesis of pathogens based on their sequence information has become a well-established procedure in science. Successful syntheses have been demonstrated for both viruses and prokaryotes. However, the technology is not yet available for routine diagnostic purposes. Due to the potential utility of diagnostic sequencing and sequence-based de novo synthesis of pathogens, it seems worthwhile to establish the technology for diagnostic purposes over the intermediate term. This is particularly true for resource-restricted deployment settings, where safe handling of harmful pathogens cannot always be guaranteed.