Articles by Xi Chen in JoVE
High-Throughput, Multi-Image Cryohistology of Mineralized Tissues Nathaniel A. Dyment1, Xi Jiang1, Li Chen1, Seung-Hyun Hong2, Douglas J. Adams3, Cheryl Ackert-Bicknell4, Dong-Guk Shin2, David W. Rowe1 1Department of Reconstructive Sciences, University of Connecticut Health Center, 2Department of Computer Science and Engineering, University of Connecticut, 3Department of Orthopaedic Surgery, University of Connecticut Health Center, 4Department of Orthopaedics, University of Rochester In this manuscript, we present a high-throughput, semi-automated cryohistology platform to produce aligned composite images of multiple response measures from several rounds of fluorescent imaging on frozen sections of mineralized tissues.
Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay Shani Leviatan Ben-Arye1, Hai Yu2, Xi Chen2, Vered Padler-Karavani1 1Department of Cell Research and Immunology, Tel Aviv University, 2Department of Chemistry, University of California-Davis A sialoglycan microarray assay can be used to evaluate anti-Neu5Gc antibodies in human sera, making it a potential high-throughput diagnostic assay for cancer and other chronic inflammation-mediated human diseases.
Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice Kai Wang*1, Xi Wang*1, Cheng-sheng Han*2, Liang-yi Chen2, Ying Luo1 1Department of Biomedical Engineering, Peking University, 2Institute of Molecular Medicine, Peking University This protocol demonstrates murine islet isolation and seeding onto a decellularized scaffold. Scaffold-supported islets were transplanted into the epididymal fat pad of streptozotocin (STZ)-induced diabetic mice. Islets survived at the transplantation site and reversed the hyperglycemic condition.