In JoVE (4)
- Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
- Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
- Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
- Photoselective Vaporesection of the Prostate via an End-firing Lithium Triborate Crystal Laser
Articles by Xin Liu in JoVE
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models Dorothy R. Ahlf Wheatcraft1,2, Xin Liu1,2, Amanda B. Hummon1,2 1Department of Chemistry and Biochemistry, University of Notre Dame, 2Harper Cancer Research Institute, University of Notre Dame Immortalized cancer cell lines can be grown as 3D cell cultures, a valuable model for biological research. This protocol describes mass spectrometry imaging of 3D cell cultures, including improvements in the sample preparation platform. The goal of this protocol is to instruct users to prepare 3D cell cultures for mass spectrometry imaging analysis.
Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy Haochuan Zhang*1,2,3, JunGang Huang*2,3,4, Xin Liu2,3, Ping Zhu4, Zhongrong Li1, Xue Li2,3 1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 2 We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.
Detection of Rare Mutations in CtDNA Using Next Generation Sequencing Xiaoxing Lv1, Meiru Zhao1, Yuting Yi1, Lucheng Zhang1, Yanfang Guan1, Tao Liu1, Ling Yang1, Rongrong Chen1, Jianhui Ma1, Xin Yi1 1Geneplus-Beijing Institute This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.
Photoselective Vaporesection of the Prostate via an End-firing Lithium Triborate Crystal Laser Xin Wang1, Yao-Guang Zhang1, Sheng-Cai Zhu1, Ben Wan1, Ming Liu1, Jian-Ye Wang1 1Department of Urology, Beijing Hospital, National Center of Gerontology Here we present a protocol to perform Photoselective Vaporesection of the Prostate (PVRP) for benign prostatic hyperplasia (BPH) treatment.
Other articles by Xin Liu on PubMed
TASK-3, a Novel Tandem Pore Domain Acid-sensitive K+ Channel. An Extracellular Histiding As PH Sensor The Journal of Biological Chemistry. Jun, 2000 | Pubmed ID: 10747866 Tandem pore domain acid-sensitive K(+) channel 3 (TASK-3) is a new member of the tandem pore domain potassium channel family. A cDNA encoding a 365- amino acid polypeptide with four putative transmembrane segments and two pore regions was isolated from guinea pig brain. An orthologous sequence was cloned from a human genomic library. Although TASK-3 is 62% identical to TASK-1, the cytosolic C-terminal sequence is only weakly conserved. Analysis of the gene structure identified an intron within the conserved GYG motif of the first pore region. Reverse transcriptase-polymerase chain reaction analysis showed strong expression in brain but very weak mRNA levels in other tissues. Cell-attached patch-clamp recordings of TASK-3 expressed in HEK293 cells showed that the single channel current-voltage relation was inwardly rectifying, and open probability increased markedly with depolarization. Removal of external divalent cations increased the mean single channel current measured at -100 mV from -2.3 to -5.8 pA. Expression of TASK-3 in Xenopus oocytes revealed an outwardly rectifying K(+) current that was strongly decreased in the presence of lower extracellular pH. Substitution of the histidine residue His-98 by asparagine or tyrosine abolished pH sensitivity. This histidine, which is located at the outer part of the pore adjacent to the selectivity filter, may be an essential component of the extracellular pH sensor.
Inhibitive Effect of Artemether on Tumor Growth and Angiogenesis in the Rat C6 Orthotopic Brain Gliomas Model Integrative Cancer Therapies. Mar, 2009 | Pubmed ID: 19174507 To explore the inhibitive effect of artemether on glioma growth and angiogenesis in brain tumor bearing SD rat. MTT assay was used to evaluate the inhibitory effect of artemether treatment on C6 glioma cells. Forty SD rats which were subcutaneous planted with SD rat C6 glioma cell to establish SD rat orthotopic glioma model were divided resourcefully into 5 groups. each group was 8 rats. Length-path (a mm) and short-path (b mm) of tumor each rat was measured. Tumor volume was calculated using the following formula: V (mm(3)) = a(2)bpi/6. Microvessel density (MVD) in different therapy groups was significantly lower than that in normal saline control group and brain glioma volume in different therapy groups was significantly smaller than that in normal saline control group. There were remarkably inhibitory effects of artmeter on brain glioma growth and angiogenesis in SD rats and the mechanism that artemether inhibited brain glioma growth might be penetrating the blood-brain barrier and inhibiting angiogenesis.
Adoptive Transfer of Pregnancy-induced CD4+CD25+ Regulatory T Cells Reverses the Increase in Abortion Rate Caused by Interleukin 17 in the CBA/JxBALB/c Mouse Model Human Reproduction (Oxford, England). May, 2014 | Pubmed ID: 24556316 Could adoptive transfer of pregnancy-induced CD4+CD25+ regulatory T cells (Tregs) reverse the increase in abortion rate caused by interleukin 17 (IL-17) in the CBA/J × BALB/c mouse model?