In JoVE (2)
Other Publications (2)
Articles by Xuejun H. Parsons in JoVE
Effektiv Utledning av Human nevrale stamceller og nevroner fra pluripotent humane embryonale stamceller med lite molekyl Induksjon Xuejun H. Parsons1,2, Yang D. Teng3,4, James F. Parsons1,2, Evan Y. Snyder1,2,5, David B. Smotrich1,2,6, Dennis A. Moore1,2 1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery, Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, 6La Jolla IVF Vi har etablert en protokoll for induksjon av neuroblasts direkte fra pluripotent humane embryonale stamceller vedlikeholdt under definerte forhold med små molekyler, som muliggjør avledning av en stor tilførsel av menneskelig neuronal stamfedre og neuronal celletyper i utviklingsland CNS for nevrale reparasjon.
Effektiv Utledning av Human Cardiac Forstadier og cardiomyocytes fra pluripotent humane embryonale stamceller med lite molekyl Induksjon Xuejun H. Parsons1,2, Yang D. Teng3,4, James F. Parsons1,2, Evan Y. Snyder1,2,5, David B. Smotrich1,2,6, Dennis A. Moore1,2 1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery, Harvard Medical School, 4Division of SCI Research, VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, 6La Jolla IVF Vi har etablert en protokoll for induksjon av cardioblasts direkte fra pluripotent humane embryonale stamceller vedlikeholdt under definerte forhold med små molekyler, som muliggjør avledning av en stor tilførsel av menneskelig hjerte stamfedre og funksjonelle cardiomyocytes for hjerte-reparasjon.
Other articles by Xuejun H. Parsons on PubMed
Behavioral Improvement in a Primate Parkinson's Model is Associated with Multiple Homeostatic Effects of Human Neural Stem Cells Proceedings of the National Academy of Sciences of the United States of America. Jul, 2007 | Pubmed ID: 17586681 Stem cells have been widely assumed to be capable of replacing lost or damaged cells in a number of diseases, including Parkinson's disease (PD), in which neurons of the substantia nigra (SN) die and fail to provide the neurotransmitter, dopamine (DA), to the striatum. We report that undifferentiated human neural stem cells (hNSCs) implanted into 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated Parkinsonian primates survived, migrated, and had a functional impact as assessed quantitatively by behavioral improvement in this DA-deficit model, in which Parkinsonian signs directly correlate to reduced DA levels. A small number of hNSC progeny differentiated into tyrosine hydroxylase (TH) and/or dopamine transporter (DAT) immunopositive cells, suggesting that the microenvironment within and around the lesioned adult host SN still permits development of a DA phenotype by responsive progenitor cells. A much larger number of hNSC-derived cells that did not express neuronal or DA markers was found arrayed along the persisting nigrostriatal path, juxtaposed with host cells. These hNSCs, which express DA-protective factors, were therefore well positioned to influence host TH+ cells and mediate other homeostatic adjustments, as reflected in a return to baseline endogenous neuronal number-to-size ratios, preservation of extant host nigrostriatal circuitry, and a normalizing effect on alpha-synuclein aggregation. We propose that multiple modes of reciprocal interaction between exogenous hNSCs and the pathological host milieu underlie the functional improvement observed in this model of PD.
Important Precautions when Deriving Patient-specific Neural Elements from Pluripotent Cells Cytotherapy. 2009 | Pubmed ID: 19903095 Multipotent human neural stem cells (hNSC) have traditionally been isolated directly from the central nervous system (CNS). To date, as a therapeutic tool in the treatment of neurologic disorders, the most promising results have been obtained using hNSC isolated directly from the human fetal neuroectoderm. The propagation ability of such tissue-derived hNSC is often limited, however, making it difficult to establish a large-scale culture. Following engraftment, these hNSC often show low efficiency in generating the desired neuronal cells necessary for reconstruction of the damaged host milieu and, as a result, have failed to give satisfactory results in clinical trials so far. Alternatively, human embryonic stem cells (hESC) offer a pluripotent reservoir for in vitro derivation of a rich spectrum of well-characterized neural-lineage committed stem/progenitor/precursor cells that can, theoretically, be picked at precisely their safest and most efficacious state of plasticity to meet a given clinical challenge. However, the need for 'foreign' biologic additives and multilineage differentiation inclination may make direct use of such cell-derived hNSC in patients problematic. The hNSC, when derived from pluripotent cells under protocols presently employed in the field, tend to display not only a low efficiency in neuronal differentiation, but also an inclination for phenotypic heterogeneity and instability and, hence, increased risk of tumorigenesis following engraftment. For hNSC derived in vitro to be used safely in therapeutic paradigms, it requires conversion of human pluripotent cells uniformly to cells that are restricted to the neural lineage in need of repair. Developing strategies for direct induction of human pluripotent cells exclusively into neural-committed progenies at a broad range of developmental stages will allow a large supply of optimal therapeutic hNSC tailor-made for safe and effective treatment of particular neurologic diseases and injuries in patients.