Other Publications (1)
Articles by Ya-Min Du in JoVE
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+ Ya-Min Du1, Shen-Li Zheng1, Li Liu1, Josef Voglmeir1, Gabriel Yedid2 1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, 2College of Life Science, Nanjing Agricultural University We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).
Other articles by Ya-Min Du on PubMed
Comparison of Anti-pathogenic Activities of the Human and Bovine Milk N-glycome: Fucosylation is a Key Factor Food Chemistry. Nov, 2017 | Pubmed ID: 28554622 Health differences between breast- and formula-fed infants have long been apparent despite great efforts in improving the function of baby formula by adjusting the levels of various milk nutritional components. However, the N-glycome, a type of oligosaccharide decorating a diverse range of proteins, has not been extensively studied in milk regarding its biological function. In this study, the anti-pathogenic function of the enzymatically released human and bovine milk N-glycome against 5 food-borne pathogens was investigated. The human milk N-glycome showed significantly higher activity than bovine milk. After enzymatic defucosylation of human and bovine N-glycan pool, UHPLC peak shifts were observed in both suggesting heavy fucosylation of samples. Furthermore, the anti-pathogenic activity of the defulosylated N-glycome decreased significantly, and the significance of functional difference between the two almost disappeared. This result indicates the essential role of fucosylation for the anti-pathogenic function of the milk N-glycome, especially in human milk.