Articles by Yasnee Beeharry in JoVE
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency Stéphanie Beaucourt1, Antonio V. Bordería1, Lark L. Coffey1, Nina F. Gnädig1, Marta Sanz-Ramos1, Yasnee Beeharry1, Marco Vignuzzi1 1Viral Populations and Pathogenesis lab and CNRS 3015, Institut Pasteur The present article describes the steps required to isolate and characterize RNA polymerase fidelity variants of RNA viruses and how to use mutation frequency data to confirm fidelity changes in tissue culture.
Other articles by Yasnee Beeharry on PubMed
Arbovirus High Fidelity Variant Loses Fitness in Mosquitoes and Mice Proceedings of the National Academy of Sciences of the United States of America. Sep, 2011 | Pubmed ID: 21896755 The error rate of RNA-dependent RNA polymerases (RdRp) affects the mutation frequency in a population of viral RNAs. Using chikungunya virus (CHIKV), we describe a unique arbovirus fidelity variant with a single C483Y amino acid change in the nsP4 RdRp that increases replication fidelity and generates populations with reduced genetic diversity. In mosquitoes, high fidelity CHIKV presents lower infection and dissemination titers than wild type. In newborn mice, high fidelity CHIKV produces truncated viremias and lower organ titers. These results indicate that increased replication fidelity and reduced genetic diversity negatively impact arbovirus fitness in invertebrate and vertebrate hosts.