In JoVE (1)
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Articles by Yonatan Shapira in JoVE
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments Rinat Arbel-Goren1, Yonatan Shapira1, Joel Stavans1 1Department of Physics of Complex Systems, Weizmann Institute of Science This manuscript describes a method for labeling individual messenger RNA (mRNA) transcripts with fluorescently-labeled DNA probes, for use in single-molecule fluorescence in situ hybridization (smFISH) experiments in E. coli. smFISH is a visualization method that allows the simultaneous detection, localization, and quantification of single mRNA molecules in fixed individual cells.