In JoVE (1)
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Articles by Youkyeong Gloria Byun in JoVE
A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes Youkyeong Gloria Byun1, Won-Suk Chung1 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis.