In JoVE (1)
Articles by Yubin Kim in JoVE
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood Yang-Seok Park1, Vijaya Sunkara1, Yubin Kim1, Won Seok Lee1,2, Ja-Ryoung Han1,3, Yoon-Kyoung Cho1,4 1Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Current Affiliation is Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3Current Affiliation is KOGAS (Korea Gas Corporation) Research Institute, Daegu, Republic of Korea, 4Center for Soft and Living Matter, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan, Republic of Korea This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.
Other articles by Yubin Kim on PubMed
Lab-on-a-disc for Simultaneous Determination of Nutrients in Water Analytical Chemistry. Mar, 2013 | Pubmed ID: 23320485 In this study, we describe a novel platform based on centrifugal microfluidics for simultaneous determination of nitrite, nitrate and nitrite, ammonium, orthophosphate, and silicate in water samples. All processes from sample metering to detection were integrated and automatically conducted on a rotating disc-shaped device. Fluid transfer was controlled by laser irradiation on the ferrowax-based microvalves. Liquid samples and reagents were pumped by centrifugal force in the rotating disc, and their positions and movements were controlled through a programmable light from a laser diode. This novel water analysis platform required only 500 μL of sample (100 μL for each nutrient) and 10-30 μL of reagents for colorimetric detection. In addition, the fully automated parallel processes and efficient mixing in the rotating disc allowed for a significant reduction in total analysis time (∼7 min 40 s) and increased accuracy. Validation with a seawater certified reference material indicated that the platform accurately measured nutrient concentrations in water samples. In addition, we showed that the nutrients in the seawater collected from Chunsu Bay in Korea measured by the proposed lab-on-a-disc and by a commercialized autoanalyzer are comparable.
Rapid and Automated Quantification of Microalgal Lipids on a Spinning Disc Analytical Chemistry. Aug, 2015 | Pubmed ID: 26121222 We have developed a fully integrated centrifugal microfluidic device for rapid on-site quantification of lipids from microalgal samples. The fully automated serial process involving cell sedimentation and lysis, liquid-liquid extraction, and colorimetric detection of lipid contents was accomplished within 13 min using a lab-on-a-disc. The presented organic solvent-tolerable (for n-hexane, ethanol) microfluidic disc was newly fabricated by combining thermal fusion bonding and carbon dot-based valving techniques. It is expected that this novel platform will possibly contribute toward sustainable biofuel applications by providing a practical solution for on-site monitoring of lipid accumulation in microalgal samples, thus providing imperative contribution toward energy and environmental purposes of centrifugal microfluidic technology.