Articles by Zhen Zeng in JoVE
A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen Yizhong Huang1, Maozhang He1, Qingjie Zeng1, Lin Li1, Zhen Zhang1, Junwu Ma1, Yanyu Duan1 1State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University This protocol describes a procedure to freeze muscle tissues by plunging them directly into liquid nitrogen. This protocol also highlights a new cryovial that can avoid the "blanket effect" of nitrogen gas when liquid nitrogen contacts the tissue surface of a specimen.
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning Cheng Qin*1,2, Yijiang Bai*1,2, Zhen Zeng1,2, Liao Wang1,2, Zhiwen Luo1,2, Shunqi Wang1,3, Suqi Zou1,3 1Institute of Life Science, Nanchang University, 2Queen Mary School, Medical Department, Nanchang University, 3School of Life Science, Nanchang University Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.