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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

November 3rd, 2023
*1, *1, 1, 2, 3, 2, 2, 2, 3, 1,2,4, 2
1Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, 3Department of Biomedical Engineering, University of Miami, 4VA Tennessee Valley Healthcare System
* These authors contributed equally

Summary
Automatic Translation

В этом протоколе описан метод синхронного сбора и совместной регистрации внутриклеточных сигнальных событий и секреции инсулина и глюкагона первичными псевдоостровками человека с использованием аденовирусной доставки циклического биосенсора аденозинмонофосфата (цАМФ), детектора разности цАМФ in situ (cADDis) и микроперифузионной системы.

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