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Epigenetic Inheritance and ChIP in
Caenorhabditis elegans
May 05, 2023
基于荧光报告基因和染色质免疫沉淀法（ChIP）分析
秀丽隐杆线虫
的跨代表观遗传
01:27 min
•
漂白和接种
秀丽隐杆线
虫P0代胚胎到RNAi-NGM平板上
02:36 min
•
秀丽隐杆线虫
P0 代种系 GFP 表达的传代和评分
02:02 min
•
用于染色质免疫沉淀 （ChIP） 的 F1 代
秀丽隐杆线
虫的收集
00:59 min
•
F1 代
秀丽隐杆线
虫的甲醛交联用于 ChIP
01:18 min
•
甲醛交联
秀丽隐杆线
虫超声处理及染色质免疫沉淀
02:41 min
•
Chengyin Li*
1
,
Phoebe A. W. Bhagoutie*
1
,
Victor Lao
,
Arneet L. Saltzman
1
Department of Cell and Systems Biology
,
University of Toronto
Summary
Automatic Translation
English (Original)
العربية (Arabic)
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français (French)
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该方案描述了RNA干扰和ChIP测定，以研究
秀丽隐杆线虫
中RNAi诱导的沉默和相关染色质修饰的表观遗传。
Article
