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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023
*1,2, *3, 4, 2, 1, 1, 1, 1, 5, 1, 1,2
1Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 2Zhejiang University School of Medicine, 3Hubei Normal University, 4National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, 5Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health
* These authors contributed equally

Summary
Automatic Translation

Este protocolo presenta un método completo y eficiente para producir organoides renales a partir de células madre pluripotentes inducidas (iPSC) utilizando condiciones de cultivo en suspensión. El énfasis principal de este estudio radica en la determinación de la densidad celular inicial y la concentración de agonistas WNT, lo que beneficia a los investigadores interesados en la investigación de organoides renales.

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