JoVE Biology Newsletter – March 2019

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Spaceflight Effects on Mouse Physiology


Dr. Sylvain V. Costes and colleagues at NASA Ames Research Center demonstrate how to carry out rodent experiments in space, and how to access collected omics data through the GeneLab platform

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Measuring Oxygen Consumption Rates in Fly Head Segments

Dr. Shahaf Peleg’s lab at Leibniz Institute for Farm Animal Biology (FBN), describes a protocol for measuring whole head oxygen consumption in a set-up resembling the physiological state to reveal new properties of drugs that may impact metabolic rates.

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Droplet-based Single Cell Transcriptomics of Adult Mammalian Tissue

Dr. Jeff Biernaskie’s lab at University of Calgary presents a method for preparing healthy adult mammalian single cells for droplet-based, high- throughput, single-cell RNA-Seq preparations.

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Raising Mexican Tetra for Whole-mount Immunohistochemistry

Dr. Clifford Tabin’s lab at Harvard Medical School demonstrates how to breed and raise Astyanax mexicanus to perform whole-mount immunostaining on post-larval fish to compare the phenotypes of surface and cave morphotypes.

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Visualizing Cellular Gibberellin Levels Using the nlsGPS1 FRET Biosensor

Dr. Alexander M. Jones’s lab at University of Cambridge demonstrates how to visualize cellular levels of the phytohormone gibberellin in Arabidopsis hypocotyls and root tips using a Förster resonance energy transfer-based (FRET) biosensor.

Immunoblotting of Cell Lines to Quantify Biomarker Proteins in Tissue Samples

Dr. David P. LeBrun’s lab at Queen’s University presents the use of quantitative immunoblotting to validate immunofluorescence histology in order to quantify a protein of interest in tissue samples for clinical and research purposes.

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Prostate Organoid Research Techniques and Methods: Three-Dimensional (3D) Culture, Spin-down, Dimpled Parafilm and Fusion Method

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Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

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Techniques for High Throughput Lifespan Measurement in Invertebrate Model Systems

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RNA/DNA In-Situ Hybridization Techniques

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Genetics

This collection focuses on genetics and incorporates five broad subdisciplines.

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Biology I: Yeast, Drosophila and C. Elegans

This collection features three model organisms commonly used in life sciences research

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Lab Safety

This collection provides safety guidelines to be followed when working with hazardous materials and equipment.

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Lab Animal Research

This collection is a comprehensive video guide for appropriate lab animal care and use.

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JoVE Science Education videos significantly improve general chemistry students’ comprehension of key concepts, as demonstrated by researchers at UCLA. Learn more about their findings in the February 2019 issue of the Journal of Chemical Education

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