CRISPR Guide RNA Cloning for Mammalian Systems
Dr. George Church’s lab, at Harvard University, describes streamlined methods for the simple, efficient and cost-effective generation of Cas9-associated guide RNAs
Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
Dr. Charles Romeo’s lab, at NIEHS, demonstrates a protocol for perforating the zona pellucida in mouse fertilized eggs with a laser to transduce embryonic cells to create transgenic mice.
Production, Crystallization, and Structure Determination of Human IKK1/α
Dr. Gourisankar Ghosh’s lab, at UCSD, describes the main steps necessary for the production and crystal structure determination of IκB Kinase 1/α.
Binding Dynamics of the Phospholipid Binding Protein Annexin A2
Dr. Ursula Rescher’s lab, at the University of Münster, presents a protocol to determine the binding affinities of annexin A2 with immobilized solid-supported bilayers by simultaneously measuring mass uptake and the viscoelastic properties.
Probing Cell Mechanics in Drosophila Embryo
Dr. Pierre-François Lenne’s lab, at Aix-Marseille Université, presents a setup of optical tweezers coupled to a light sheet microscope, to probe cell mechanics without beads in the Drosophila embryo.
High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
Dr. Todd Cole’s lab, at AbbVie, presents a protocol to measure the transit time of fluorescently labeled food through the gut of larval zebrafish in a high throughput fashion.
Prostate Organoid Research Techniques and Methods: Three-dimensional (3D) Culture, Spin-down, Dimpled Parafilm, and Fusion Method
Explore Methods Collection
Protein-Protein Interaction Methods
Explore Methods Collection
Techniques for high throughput lifespan measurement in invertebrate model systems
Explore Methods Collection
Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control
Explore Methods Collection
Neuroscience
This Sci Ed collection provides an introduction to the field of neuroscience, exploring five major branches of study.
Developmental Biology
This Sci Ed collection introduces the field of developmental biology and covers five major areas.
Lab Safety
This Sci Ed collection provides safety guidelines to be followed when working with hazardous materials and equipment.
Lab Animal Research
This Sci Ed collection is a comprehensive video guide for appropriate lab animal care and use.
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