Characterization of Plasma Membrane Rupture
Dr. Tudor Moldoveanu’s lab, at St. Jude Children’s Research Hospital, demonstrates methods for characterization of MLKL-mediated plasma membrane rupture in necroptosis.
Cell-based Assay for Myosin II Contractility
Dr. Derek A. Applewhite’s lab, at Reed College, describes a cell-based assay to investigate non-muscle myosin II contractility via the Fog signaling pathway in Drosophila S2R+ cells
CRISPR/Cas9-generated Gene Knockouts in Zebrafish
Dr. Joseph Ogas’s lab, at Purdue University, demonstrates a protocol for generation and identification of CRISPR-derived nonsense alleles and identification of mutations using next-generation sequencing.
Protein Tagging in HiPSCs Using CRISPR
Dr. Ruwanthi Gunawardane’s lab, at Allen Institute for Cell Science, describes a method for endogenous protein tagging in human induced pluripotent stem cells using CRISPR/Cas9.
Proteomic and miRNA Analysis of Polysomes
Dr. Roberta Gottlieb’s lab, at Cedars-Sinai Medical Center, demonstrates a protocol to perform polysome profiling on the isolated perfused mouse heart after Langendorff perfusion.
Rearing of Stable Flies
Dr. David Taylor’ lab, at ARS-USDA, demonstrates a procedure for rearing stable flies (Stomoxys calcitrans) in the laboratory.
Responses of Livestock and Wildlife Species to Zoonotic Bacteria
Accelerating the Development of Extracellular Vesicles for Therapeutic Use
Methods to Measure Functional Beta Cell Mass
Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control
Genetics
This Sci Ed collection focuses on genetics and incorporates five broad subdisciplines.
Cell Biology
This Sci Ed collection provides a glimpse into the field of cell biology and profiles five important cellular phenomena.
This Sci Ed collection features three model organisms commonly used in life sciences research; also covering methodology to maintain them in the laboratory.
Biology II: Mouse, Zebrafish, and Chick
This Sci Ed collection features three vertebrate species commonly used in life sciences research; also covering methodology on how they are maintained in the laboratory.
Considering a career outside of academia? Where to start.
5 Steps For A More Productive Ph.D
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