Encyclopedia Of Experiments

Urinary Tract Cancer

This collection features protocols relating to cancers originating in the regions or organs of the urinary or renal system. The techniques include organs, tissue, and cell harvesting procedures, organotypic culturing approaches, in vivo cancer modeling studies, decellularized tissue matrix generation, stem cell-related and electrophysiology studies, genetic-engineering strategies, tissue staining and imaging techniques, and protein expression analysis.

Encyclopedia Of Experiments

Urinary Tract Cancer

This collection features protocols relating to cancers originating in the regions or organs of the urinary or renal system. The techniques include organs, tissue, and cell harvesting procedures, organotypic culturing approaches, in vivo cancer modeling studies, decellularized tissue matrix generation, stem cell-related and electrophysiology studies, genetic-engineering strategies, tissue staining and imaging techniques, and protein expression analysis.

Sections

In vitro studies

In vivo studies

Ex vivo studies

In vitro studies

In vivo studies

Ex vivo studies

In vitro studies

Exosome Isolation

A Density-based Ultracentrifugation Technique Using Sucrose Cushion to Separate Exosomes from Conditioned Media of Cultured Cells

siRNA Encapsulation into Exosomes Using Electroporation

An Electric Pulse Based Technique to Load siRNA into Exosomes

Solid Phase Microextraction Sampling

A Probe-based Technique for Sample Extraction from Graft Tissues

Amphotericin-B Mediated Perforated Patch-Clamp Analysis

An Electrophysiological Technique to Study Ionic Currents in Urinary Bladder Cells

Preparation of Cancer Spheroid Blocks by Flash Freezing

A Procedure To Preserve Cancer Spheroids in Frozen Matrix Blocks

Deriving Human Kidney Podocytes from Induced Pluripotent Stem Cells

A Procedure for Directed Differentiation of Mature Kidney Podocytes from Stem Cells

Detrusor Smooth Muscle Cell Isolation

An Enzymatic Digestion Procedure to Obtain DSM Cells from Human Urinary Bladder Specimens

Vibratome Sectioning of Whole Murine Kidney

A Technique to Generate Thin Sections of Murine Kidney

Extracellular DNA Staining

A Method to Identify Extracellular DNA in FFPE Tissue Sections

Glomeruli Isolation

A Technique to Obtain Intact Glomeruli from Murine Kidney

Murine Renal Cell Isolation

A Technique to Isolate and Generate Primary Culture of Renal Tubular Epithelial Cells

Periodic Acid Schiff or PAS Staining

A Method to Assess the Structural Deformities of Glomerulus

Reverse Phase Protein Arrays Based Protein Expression Analysis

A Procedure for Simultaneous Quantification of Expression of Multiple Proteins from Cell Lysate

Expansion Pathology

A Method for High-resolution Optical Imaging of Clinical Tissue Samples

In vivo studies

Generating Renal Cell Carcinoma Model

A Protocol to Develop Orthotopic RCC Murine Model by Intrarenal Implantation of Cancer Cells

Bladder Tumor Organoid Model

A Method for Orthotopic Transplantation of Bladder Organoids into Recipient Murine Bladder

Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo

A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo

Patient-derived Tumor Xenografting

A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma

Modeling Orthotopic Bladder Tumor in Mouse Model

A Procedure for Intravesical Administration of Cancer Cells into Murine Bladder

Unilateral Ureteral Obstruction Murine Model

A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models

Urine Depletion and Bladder Rinsing in Mouse Model

A Catheter-based Procedure to Empty and Wash Murine Urinary Bladder

Delivery of DNA Plasmids into Urothelium of Mouse Bladder

An Electroporation-based Procedure to Efficiently Deliver DNA Plasmids into Urothelial Cells of Murine Bladder to Generate Genetically Engineered Models

Hydrodynamic Renal Pelvis Injection

A Technique to Directly Deliver DNA into Kidney Cells of Murine Model

Intrarenal Drug Delivery in Rats

A Method to Deliver Therapeutic Drugs in Rat Kidney via Intra-arterial Injection

Fluid Aspiration from Mouse Glomeruli

A Method to Aspirate Glomerular Fluid from Mouse Using Two-Photon Mediated Micropuncture

Ex vivo studies

Preparation of Decellularized Rat Kidney

A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney

Extracting Intact Kidney from Mouse

A Technique to Obtain Kidney Without Renal Capsule from Murine Models

Depigmentation and Prestaining of Zebrafish Kidney

A Technique to Prepare Adult Zebrafish Kidney for Staining

Kidney Flat Mount Preparation

A Procedure to Dissect and Prepare a Flat Mount for Microscopic Analysis of Adult Zebrafish Kidney

Renal Organotypic Culture on Chicken CAM

A Technique for Transplantation of Murine Kidney Explants on Chicken CAM to Induce Formation of Renal Vascular Architecture